Detection of PCV2d in pig industry of the Iberian Peninsula.

Porcine circovirus type 2 (PCV2) is a major viral pathogen affecting swine worldwide, causing significant economic losses through its association with porcine circovirus-associated diseases (PCVD), which can lead to reproductive failure, growth retardation, and increased mortality in affected herds. Although vaccination against the PCV2a and PCV2b genotypes has reduced clinical disease, the PCV2d genotype is increasingly prevalent in many regions. Molecular detection was performed on fecal samples from collected from a slaughterhouse using PCR targeting the ORF2 gene to detect PCV2 genotypes.

Role of Phospholipids on Drug Dissolution in Polymer Solid Dispersions Prepared by Hot-Melt Extrusion.

The development of solid dispersions (SDs) has gained increased attention in recent decades, leading to successful delivery systems for various marketed products. In general, combinations of polymers and lipids in SD formulations have shown promising results in reducing the disadvantages associated with the isolated use of hydrophilic excipients such as copovidone (COP) and Soluplus (SLP). This study investigated the influence of the phospholipid (soy phosphatidylcholine, PPC, 15 and 30%, w/w) on the dissolution performance of drug-polymer SDs prepared by hot melt extrusion.

Effect of DSM 32315 in Diets on Performance and Gut Integrity of Post-Weaning Piglets.

This study evaluated the effect of the replacement of AGPs by a probiotic in diets for piglets in the nursery stage. The dietary treatments were as follows: CON-basal diet (BD); ANT-BD+antibiotic; and PRO-BD+probiotic. From d 35-42 of age, the piglets that received ANT-BD showed a higher average daily weight gain (ADWG) ( = 0.

Assessing Q Fever Exposure in Veterinary Professionals: A Study on Seroprevalence and Awareness in Portugal, 2024.

Due to their frequent contact with animals, veterinarians may be at preferential risk of exposure due to occupational contact with livestock. This study assesses the seroprevalence and risk factors associated with seropositivity in Portuguese veterinarians. A cross-sectional study compared IgG anti- in veterinarians' sera to a demographically matched control group.

Airborne Fungal Spore Diversity Assessment Using Culture-Dependent and Metabarcoding Approaches in Bat-Inhabited Natural and Anthropogenic Roosts in Portugal.

Cave environments represent extreme and underexplored ecosystems wherein fungi play a crucial role in nutrient cycling and ecological dynamics. This study provides the first comprehensive assessment of fungal diversity in air samples from caves across Portugal, with six samples from five locations being assessed through culture-dependent and metabarcoding approaches. From the five bat roosts studied, eleven morphologically distinct fungal colonies were isolated, with genera such as , , and identified.

Targeted-Amplicon NGS for sp. in Shepherd Dogs of Portugal Discriminates Co-Colonization with Multiple Zoonotic Subtypes.

Research on endoparasites in pet dogs has been growing, but shepherd dogs have largely been overlooked. These dogs frequently share close proximity not only with sheep, which are reservoirs of zoonotic subtypes of , but also with their owners. This close contact increases the potential for shepherd dogs to act as intermediates in the transmission of .

Screening for the ciliate Buxtonella sulcata in free-ranging dairy cattle on Terceira Island, Azores archipelago.

Buxtonella sulcata is an alveolate ciliate protist, historically considered a commensal of the gastrointestinal tract of cattle. Despite the fact that its cysts are morphologically identical to those of Balantioides coli, molecular identification techniques have shed new light on its role as a pathogen. This work aimed to assess the presence of this ciliate in the population of dairy cattle on the Azorean island of Terceira by means of molecular analyses (ITS1-5.

First Molecular Detection of and in Ticks from Dogs in Luxembourg.

Vector-borne diseases, particularly those caused by species, pose a significant public health threat in Europe. Despite extensive research on tick-borne pathogens in various European countries, Luxembourg has yet not been studied for spp. in ticks infesting domestic animals.

Screening Wild Birds for Tick-Borne Zoonotic Pathogens in Portugal.

Wild birds may be involved in the transmission of agents of infectious diseases, including zoonoses, a circumstance which raises a number of public and animal health issues. Migratory bird species play a significant role in the introduction of tick-borne pathogens to new geographic areas, contributing to the dissemination of various etiological agents. This preliminary study aimed to assess the occurrence of four potentially zoonotic pathogens ( spp.

First Report on the Frequency and Subtype Distribution of sp. in Extensively Reared Holstein-Friesian Cattle from Terceira Island, Azores Archipelago, Portugal.

This study is the first to report sp. in dairy cattle from Terceira Island, part of the Azores Archipelago. sp.

Addressing Gearbox Health Monitoring Challenges for Helicopters: A Machine Learning Approach.

The transmission gearbox of military helicopters, such as the H225M, experiences intense dynamic loads, leading to the detachment of ferromagnetic particles, often due to wear or fatigue. This poses safety risks, as excessive particle detachment demands stringent maintenance. To address this, the study applies machine learning algorithms to predict particle detachment using data from the Flight Data Recorder and Health and Usage Monitoring System.

Multicentric Study on Enteric Protists Occurrence in Zoological Parks in Portugal.

Parasitic infections of the gastrointestinal tract of domestic animals play a major role in the transmission of disease, which in turn may result in financial and productive losses. Notwithstanding, studies on the burden and distribution of diarrheagenic protists in zoological gardens are still insufficient. Given the close animal-animal and animal-human interaction in these settings, Public Health concerns under the One Health context are raised.

Detection of in Non-Human Primates in Portuguese Zoos.

, an intracellular eukaryote closely related to fungi, is recognized as a significant pathogen affecting humans, particularly those with compromised immune systems. While its transmission routes are still not fully elucidated, fecal-oral transmission remains the primary one. With a wide host range, the zoonotic potential of is a concern, albeit direct evidence of animal-to-human transmission remains scarce.

On the faecal detection of Halicephalobus gingivalis in equines in Iran.

The life-cycle of the parasitic forms of Halicephalobus gingivalis is yet to be fully understood. In cases where there is evidence to support the presence of eggs from this parasite or its DNA in the faeces of equines, a thorough investigation of the clinical status and gastro-intestinal tract of the affected animals is warranted, as well as detailed descriptions of the employed coprological technique. Since reports of the identification of H.

Multivariate Analysis of Solubility Parameters for Drug-Polymer Miscibility Assessment in Preparing Raloxifene Hydrochloride Amorphous Solid Dispersions.

The success of obtaining solid dispersions for solubility improvement invariably depends on the miscibility of the drug and polymeric carriers. This study aimed to categorize and select polymeric carriers via the classical group contribution method using the multivariate analysis of the calculated solubility parameter of RX-HCl. The total, partial, and derivate parameters for RX-HCl were calculated.

Postoperative pain after single-visit root canal treatments in necrotic teeth comparing instruments' kinematics and apical instrumentation limits - a prospective randomized multicenter clinical trial.

Objectives: This prospective randomized multicenter clinical trial (PRMCT) investigated postoperative pain after single-visit root canal treatments in teeth affected by pulp necrosis (PN), and asymptomatic apical periodontitis (AAP) (with apical radiolucent areas) or normal periradicular tissues (without apical radiolucent areas) comparing different instruments' kinematics and apical instrumentation limits.

Methods: Before chemomechanical preparation, 240 patients/teeth were randomly distributed into four groups (n = 60) according to the instruments' kinematics (rotary or reciprocating) and apical instrumentation limits (with or without intentional foraminal enlargement [IFE]). After that, specimens were submitted to the same irrigation and obturation techniques, and the patients were referred to undergo the definitive restorations.

Kinematic and dynamic data from a robotic assembly of aeronautical threaded fasteners.

Industrial screwing is one of several industry branches' most common manufacturing processes. Good quality and structured data from these operations have increased demand with the popularization of data-driven techniques for manufacturing automation. The dataset presented in this paper comprises screwing experiments with aeronautical nuts performed by an industrial robot Kuka KR-16 in a lab setting.

Tau liquid-liquid phase separation is modulated by the Ca -switched chaperone activity of the S100B protein.

Aggregation of the microtubule-associated protein tau is implicated in several neurodegenerative tauopathies including Alzheimer's disease (AD). Recent studies evidenced tau liquid-liquid phase separation (LLPS) into droplets as an early event in tau pathogenesis with the potential to enhance aggregation. Tauopathies like AD are accompanied by sustained neuroinflammation and the release of alarmins at early stages of inflammatory responses encompass protective functions.

Tetramerization of the S100B Chaperone Spawns a Ca Independent Regulatory Surface that Enhances Anti-aggregation Activity and Client Specificity.

Alzheimer's disease (AD) hallmarks include the aggregation of amyloid-β (Aβ), tau and neuroinflammation promoted by several alarmins. Among these is S100B, a small astrocytic homodimeric protein, upregulated in AD, whose multiple biological activities depend on localization, concentration, and assembly state. S100B was reported to inhibit the aggregation and toxicity of Aβ42 and tau similarly to a holdase-type chaperone.

Correlation between short-term blood pressure variability parameters with mobil-O-graph pulse wave velocity.

Background: Blood pressure variability (BPV) and arterial stiffness show an association with increased cardiovascular events. Evidences demonstrated an association between higher short-term systolic BPV and stiffer arteries. There is no previous study assessed the correlation between BPV and arterial stiffness measured by a Mobil-O-Graph device.

Dynamic interactions and Ca-binding modulate the holdase-type chaperone activity of S100B preventing tau aggregation and seeding.

The microtubule-associated protein tau is implicated in the formation of oligomers and fibrillar aggregates that evade proteostasis control and spread from cell-to-cell. Tau pathology is accompanied by sustained neuroinflammation and, while the release of alarmin mediators aggravates disease at late stages, early inflammatory responses encompass protective functions. This is the case of the Ca-binding S100B protein, an astrocytic alarmin which is augmented in AD and which has been recently implicated as a proteostasis regulator, acting over amyloid β aggregation.

Evaluation of three methods of suture for skin closure in total knee arthroplasty: a randomized trial.

Background: There are several studies comparing techniques and different materials, yet the results are not unanimous. We compared three methods of skin closure in total knee arthroplasty (TKA), including suture with single stitches and unabsorbable MonoNylon®, as well as continuous subcuticular suture with Monocryl® or barbed Stratafix® absorbable suture.

Methods: A prospective, randomized study was conducted with 63 patients undergoing TKA between March 2016 and December 2016.

Genetic divergence for adaptability and stability in sugarcane: Proposal for a more accurate evaluation.

The best agro-industrial performance presented by a crop genotype in one environment may not be reproduced in another owing to complex edaphoclimatic variations. Therefore, breeding programs are constantly attempting to obtain, through artificial hybridization, novel genotypes with high adaptability and stability potential. The objective of this study was to analyze genetic divergence in sugarcane based on the genotypic values of adaptability and stability.

Cu-binding to S100B triggers polymerization of disulfide cross-linked tetramers with enhanced chaperone activity against amyloid-β aggregation.

S100B is an extracellular protein implicated in Alzheimer's Disease and a suppressor of amyloid-β aggregation. Herein we report a mechanism tying Cu2+ binding to a change in assembly state yielding disulfide cross-linked oligomers with higher anti-aggregation activity. This chemical control of chaperone function illustrates a regulatory process relevant under metal and proteostasis dysfunction as in neurodegeneration.