Cell Partitioning Design for Microfluidic ATPS Devices: A Dynamic Energy Strategy and Calculation Using Chondrocytes and Model Microparticles.

Sorting and isolating specific cells from heterogeneous populations are crucial for many biomedical applications, including drug discovery and medical diagnostics. Conventional methods such as Fluorescent Activated Cell Sorting (FACS) and Magnetic Activated Cell Sorting (MACS) face limitations in throughput, cost, and the ability to separate subtly different cells. Cell partitioning in Aqueous Two-Phase Systems (ATPSs) offers a biocompatible and cost-effective alternative, particularly when combined with continuous-flow microfluidics.

Ecotoxicological impact of residual polycyclic aromatic hydrocarbon pollution in the largest Brazilian marine protected area: A multibiomarker approach in the marine clam Tivela mactroides.

An unprecedented oil spill occurred along the Northeast Coast of Brazil in late 2019, affecting the Environmental Protection Area Costa dos Corais (APACC), the largest Brazilian marine conservation unit, posing a risk to this complex ecosystem. By applying passive ecotoxicological biomonitoring, this study aimed to evaluate whether the remaining contamination from such an oil spill affected the population of the marine clam Tivela mactroides by assessing multiple biomarkers, regarding antioxidant enzyme activities (SOD, CAT), biotransformation (GST, GSH), oxidative damage (MDA), and neurotoxicity (AChE) in gills and digestive glands, and PAH bioaccumulation. The clam tissues and sediment were sampled in three locations (Paripueira, Japaratinga and Maragogi beaches), which were differently affected by the accident, and during three sampling periods, starting one year after the accident began.

3D polymerization (-3DP): Implementing an aqueous two-phase system for the formation of 3D objects inside a microfluidic channel.

Rapid prototyping and fabrication of microstructure have been revolutionized by 3D printing, especially stereolithography (SLA) based techniques due to the superior spatial resolution they offer. However, SLA-type 3D printing faces intrinsic challenges in multi-material integration and adaptive Z-layer slicing due to the use of a vat and a mechanically controlled Z-layer generation. In this paper, we present the conceptualization of a novel paradigm which uses dynamic and multi-phase laminar flow in a microfluidic channel to achieve fabrication of 3D objects.