Detection of HPV-16 by a Simple and Cost-Effective DNA Probe: Polyadenine-Polythymine-Decorated Gold Nanoparticles (PolyA-PolyT@AuNPs).

HPV-16 identification is crucial for point-of-care molecular diagnosis of cervical cancer. However, the diagnostic methods currently in use are unable to combine both high analytical performance and cost-efficient medical diagnosis. To circumvent this, we have developed a novel nanobiosensor for diagnosis of the HPV-16 L1 gene sequence through colorimetric methods.

Highly Efficient Production of Benzene-Free Aromatics from Methanol over Low-Si/Al-Ratio Alkali-Modified Fe/Zn/HZSM-5.

Herein, the methanol conversion to aromatic hydrocarbons was studied over a new family of mesoporous low-silica HZSM-5 (Si/Al = 11) catalysts in a fixed-bed tubular reactor under ambient pressure at 375 °C, feeding with weight hourly space velocity of 2 h. The catalysts were prepared in the absence and presence of Zn and Fe in both alkaline and neutral aqueous solutions, characterized by using X-ray diffraction, X-ray fluorescence, temperature programmed desorption of ammonia, N adsorption/desorption, thermogravimetric analysis, Fourier-transform infrared, transmission electron microscopy (TEM), field emission scanning electron microscopy and FE-SEM/energy dispersive X-ray spectroscopy techniques. The [0.

Induction of Apoptosis by Extract of Persian Gulf Marine Mollusk, through the ROS-Mediated Mitochondrial Targeting on Human Epithelial Ovarian Cancer Cells.

Despite recent improvements in treatment, ovarian cancer is still the leading cause of death from gynaecological malignancies. Today, marine mollusks are considered as natural source of new biologically and pharmacologically active compounds by scientists and the pharmaceutical industries. The aim of this study is to investigate the selective apoptotic effects of crude extract fractions on human epithelial ovarian cancer (EOC) cells and mitochondria.

Induction of Apoptosis by an Extract of Persian Gulf Marine Mollusc, Turbo Coronatus through the Production of Reactive Oxygen Species in Mouse Melanoma Cells.

Objective: A variety of approaches such as surgery, chemotherapy, radiotherapy, hormonal therapy and immunotherapy are used to treat melanomas, but unfortunately in most case, the response is very weak and often side effects are serious. This study concerns selective toxicity of an extract of Turbo coronatus on cells and mitochondria from a syngeneic mouse model of melanoma. Methods: Cells and mitochondria isolated from extra tumoral and melanoma tissues were exposed toa T.

Naja Naja Oxiana Venom Fraction Selectively Induces ROS-Mediated Apoptosis in Human Colorectal Tumor Cells by Directly Targeting Mitochondria.

Objective: To investigate the selective effect of Naja naja oxiana crude venom and its fractions on human colorectal cancer mitochondria to activate apoptosis signaling. Methods: Cells and mitochondria isolated from human cancerous and normal colorectal tissues exposed to N. oxiana crude venom and its fractions obtained from size-exclusion chromatography and then mitochondrial parameters related to up-stream cell death signalling such as reactive oxygen species formation, MMP, mitochondrial swelling, cytochrome c release and ATP content as mitochondrial parameters and activation of caspase3 and finally apoptosis/necrosis % were then assayed as cellular parameters.

Persian Gulf Jellyfish (Cassiopea andromeda) Venom Fractions Induce Selective Injury and Cytochrome C Release in Mitochondria Obtained from Breast Adenocarcinoma Patients.

Objective: This study was conducted to investigate whether fractions of jellyfish Cassiope andromeda venom, could selectively induce toxicity on mitochondria isolated from cancer tissue of patients with breast adenocarcinomas. Methods: Firstly, we extracted two fractions, (f1 and f2) from crude jellyfish venom by gel filtration on Sephadex G-200.Then different dilutions of these extracted fractions were applied to mitochondria isolated from human breast tumoral- and extra-tumoral tissues.