Publications by authors named "Eve G D Hopkins"
Citrobacter rodentium Infection Induces Persistent Molecular Changes and Interferon Gamma-Dependent Major Histocompatibility Complex Class II Expression in the Colonic Epithelium.
mBio
February 2021
Article Synopsis
- Most studies on mucosal infections have primarily focused on the acute phase, leaving gaps in knowledge about tissue recovery and long-term effects post-infection.
- Researchers conducted a detailed proteomic analysis on colonic epithelial cells from infected mice, revealing significant inflammatory responses during the clearance phase and persistent changes, including an ongoing immune response four weeks post-clearance.
- Despite the presence of these long-term immune changes, both wild-type and specific immune-deficient mice exhibited similar protective responses against future infections, indicating that certain immune mechanisms may not be crucial for protection.
The gut microbiota plays a crucial role in susceptibility to enteric pathogens, including Citrobacter rodentium, a model extracellular mouse pathogen that colonizes the colonic mucosa. C. rodentium infection outcomes vary between mouse strains, with C57BL/6 and C3H/HeN mice clearing and succumbing to the infection, respectively.
View Article and Find Full Text PDFOverview of the Effect of Citrobacter rodentium Infection on Host Metabolism and the Microbiota.
Methods Mol Biol
April 2021
Citrobacter rodentium is a natural enteric mouse pathogen that models human intestinal diseases, such as pathogenic E. coli infections, ulcerative colitis, and colon cancer. Upon reaching the monolayer of intestinal epithelial cells (IECs) lining the gut, a complex web of interactions between the host, the pathogen, and the microbiota ensues.
View Article and Find Full Text PDFArticle Synopsis
- Citrobacter rodentium serves as a valuable mouse model for studying infections caused by EHEC and EPEC, particularly in understanding severe colitis responses in C3H/HeN mice.
- Infection in these mice leads to rapid colonization, inflammation, and changes in energy metabolism, marked by a shift from oxidative phosphorylation to aerobic glycolysis.
- The research highlights unique responses in C3H/HeN mice, such as enhanced activity of specific patterns recognition receptors and the activation of the ALPK1/TIFA signaling pathway, offering insights into severe infectious colitis similar to EPEC infections in humans.
Citrobacter rodentium is an extracellular enteric mouse-specific pathogen used to model infections with human pathogenic Escherichia coli and inflammatory bowel disease. C. rodentium injects type III secretion system effectors into intestinal epithelial cells (IECs) to target inflammatory, metabolic and cell survival pathways and establish infection.
View Article and Find Full Text PDFWe used the mouse attaching and effacing (A/E) pathogen , which models the human A/E pathogens enteropathogenic and enterohemorrhagic (EPEC and EHEC), to temporally resolve intestinal epithelial cell (IEC) responses and changes to the microbiome during infection. We found the host to be unresponsive during the first 3 days postinfection (DPI), when resides in the caecum. In contrast, at 4 DPI, the day of colonic colonization, despite only sporadic adhesion to the apex of the crypt, we observed robust upregulation of cell cycle and DNA repair processes, which were associated with expansion of the crypt Ki67-positive replicative zone, and downregulation of multiple metabolic processes (including the tricarboxylic acid [TCA] cycle and oxidative phosphorylation).
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