Publications by authors named "Erica Margiotta"
Changing environmental conditions necessitate rapid adaptation of cytoplasmic and nuclear volumes. We use the slime mold Dictyostelium discoideum, known for its ability to tolerate extreme changes in osmolarity, to assess which role nuclear pore complexes (NPCs) play in achieving nuclear volume adaptation and relieving mechanical stress. We capitalize on the unique properties of D.
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- The eukaryotic nucleus is surrounded by a nuclear envelope with nuclear pore complexes (NPCs) that allow for transport between the nucleus and cytoplasm, made up of around 1000 proteins from about 30 different nucleoporins.
- The architecture of the NPC is dynamic, capable of changing shape in response to external forces, which complicates efforts to understand its function at a detailed structural level.
- Recent advancements in structural biology, including in situ techniques and proteomic analysis, are helping researchers create more accurate models of the NPC, despite challenges like relying on structures from different species.
Human immunodeficiency virus (HIV-1) remains a major health threat. Viral capsid uncoating and nuclear import of the viral genome are critical for productive infection. The size of the HIV-1 capsid is generally believed to exceed the diameter of the nuclear pore complex (NPC), indicating that capsid uncoating has to occur prior to nuclear import.
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