Development of an RT-LAMP Assay for Detecting (M) in Species: Enhancing AMR Surveillance Within the One Health Sectors.

The increasing prevalence of antimicrobial-resistant (AMR) bacteria in humans, animals, and the environment underscores the necessity for a rapid, sensitive, and specific method to identify resistance genes. : This study aims to develop a reliable detection tool for identifying the tetracycline-resistant gene (M) in species using a real-time loop-mediated isothermal amplification (RT-LAMP) assay. Real-time visualization through a turbidimeter enabled precise estimation of time-to-positivity for gene detection.

Immune stimulatory effect of in healthy animal models: A systematic review and meta-analysis.

The immune-modulatory effects of black seeds ( seeds, NSS) are well documented, but the overall impact of this important natural medicinal product on immune system function has yet to be established. Here we systematically reviewed and meta-analyzed the effects of NSS on humoral [serum titers of immunoglobulins including IgG, IgM, anti-Newcastle virus disease (anti-NDV), and sheep red blood cell antigen (anti-SRBC)] and cellular immunity [total white blood cell (WBC) count and percentages of monocytes, lymphocytes, basophils, neutrophils, and eosinophils] in healthy animals. The PubMed, ScienceDirect, Web of Science, and Scopus databases were searched according to predefined eligibility criteria.