Publications by authors named "Dmitriy A Karagodin"
Chromosomal inversions play a crucial role in evolution and have been found to regulate epidemiologically significant traits in malaria mosquitoes. However, they have not been characterized in Aedes aegypti, the primary vector of arboviruses, due to the poor structure of its polytene chromosomes. The Hi-C proximity ligation approach was used to identify chromosomal inversions in 25 strains of A.
View Article and Find Full Text PDFIn this study, we sampled malaria mosquito larvae in natural and artificial breeding places to study the geographical distribution, ecological preferences, and chromosomal variability of different species of the genus in the territory of the Crimean Peninsula and the Black Sea coast of the Caucasus. Species were diagnosed using a combination of morphological, cytogenetic, and molecular markers. The ecological conditions of the larval habitats, such as dissolved oxygen content in the water, acidity, salinity, and temperature, were measured.
View Article and Find Full Text PDFBackground: Understanding genome organization and evolution is important for species involved in transmission of human diseases, such as mosquitoes. Anophelinae and Culicinae subfamilies of mosquitoes show striking differences in genome sizes, sex chromosome arrangements, behavior, and ability to transmit pathogens. However, the genomic basis of these differences is not fully understood.
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- GAGA (GAF) is a transcription factor crucial for regulating gene expression during fly development, and its underexpression in mutants leads to significant germline cell death and reduced testis size.
- Our RNA-seq analysis identified 2,437 differentially expressed genes in GAGA underexpressed testes, revealing downregulation of normal testis genes and an increase in stress-related genes.
- The study concludes that GAGA deficiency causes metabolic imbalances and impaired mitochondrial function, contributing to cellular stress and sperm cell death, but no specific GAGA-dependent cell death pathway was identified.
Article Synopsis
- The study investigates the genetic diversity of the Eurasian malaria vector, highlighting five significant chromosomal inversions found in its natural populations.
- Researchers identify a cryptic species distinguished by nucleotide substitutions in the ribosomal DNA's Internal Transcribed Spacer 2 (ITS2) region, though a complete reference cytogenetic map is lacking.
- A new cytogenetic map and ITS2-RFLP diagnostic method were developed, revealing notable differences in chromosomal inversion frequencies between the two species in a population from Western Siberia, with the main species exhibiting greater inversion polymorphism.
Article Synopsis
- * Researchers analyzed 276 specimens from various U.S. locations and created a reliable method using ITS2 sequences for species identification, leading to a phylogenetic tree of Anopheles species.
- * Findings showed consistent ITS2 sequence length without variation, indicating An. punctipennis is a single species across the examined areas, and identified its close relationship with other mosquitoes in the Maculipennis complex.
Article Synopsis
- - Long-read sequencing technologies are enhancing research on mosquito genomes, helping scientists understand how these insects transmit diseases.
- - Cytogenetic mapping using fluorescence in situ hybridization (FISH) is vital for connecting genomic data to specific chromosomes, especially in complex genomes with repetitive DNA.
- - This study introduces a new method for gene-based physical mapping, which successfully uses complementary DNA and specific protein-coding genes to create accurate chromosome maps for mosquitoes, with potential applications in other insect species.
Article Synopsis
- * A study analyzed the genetic differences between two closely related species, focusing on specific nucleotide substitutions and inversion frequencies in 289 larvae from the Moscow region.
- * Results showed significant genomic divergence, especially on the inversion-rich X chromosome, indicating that inversions contribute to the limited reproductive isolation between these two species.