Circulating miRNAs as potential liquid biomarkers for pediatric gliomas.

Background: Current diagnostic and monitoring of pediatric brain tumors rely on invasive tissue biopsies and imaging, highlighting the need for non-invasive alternatives. For that matter, liquid biopsy is a promising method. This pilot study investigated the potential of circulating miRNAs to serve as non-invasive biomarkers of pediatric glioma tumors.

Patient-Derived Gastric Cancer Assembloid Model Integrating Matched Tumor Organoids and Stromal Cell Subpopulations.

: Conventional three-dimensional in vitro tumor models often fail to fully capture the complexity of the tumor microenvironment, particularly the diverse populations of cancer-associated fibroblasts that contribute to poor prognosis and treatment resistance. The purpose of this study is to develop a patient-specific gastric cancer assembloid model that integrates tumor epithelial cells with matched stromal cell subtypes, each derived using tailored growth media to enhance cancer preclinical research and advance personalized therapeutic strategies. : Tumor tissue was dissociated, and cells expanded in media for organoids, mesenchymal stem cells, fibroblasts, or endothelial cells.