GATA3-Controlled Nucleosome Eviction Drives Enhancer Activity in T-cell Development and Leukemia.

Long-range enhancers govern the temporal and spatial control of gene expression; however, the mechanisms that regulate enhancer activity during normal and malignant development remain poorly understood. Here, we demonstrate a role for aberrant chromatin accessibility in the regulation of expression in T-cell lymphoblastic leukemia (T-ALL). Central to this process, the NOTCH1- enhancer (N-Me), a long-range T cell-specific enhancer, shows dynamic changes in chromatin accessibility during T-cell specification and maturation and an aberrant high degree of chromatin accessibility in mouse and human T-ALL cells.