Exploring the role of microbiota in mediating sexually dimorphic infection outcomes in mealworm beetles.

Sexually dimorphic responses to pathogenic infections in animals may stem from sex-specific differences in their life history and immune investment. Recent evidence highlights that such sex-specific variations in immune responses can also be critically regulated by the microbiota. However, direct experiments to test how the microbiota jointly impacts sex-specific immunity and vulnerability to pathogens are still limited.

Effect of developmental and adult diet composition on reproductive aging in Drosophila melanogaster.

Diet significantly affects reproductive outcomes across species, yet the precise effects of macronutrient compositions beyond caloric intake on reproductive aging are understudied. Existing literature presents conflicting views on the fertility impacts of nutrient-rich versus nutrient-poor developmental diets, underscoring a notable research gap. This study addresses these gaps by examining effects of isocaloric diets with varied protein-to-carbohydrate ratios during both developmental and adult stages on reproductive aging of a large, outbred Drosophila melanogaster population (n = ∼2100).

Non-protein A purification platform for continuous processing of monoclonal antibody therapeutics.

Protein A capture chromatography, the core of a mAb purification platform, is known to account for more than 50% of downstream processing costs along with other limitations including lack of complete stability to alkaline cleaning solutions, relatively lower binding capacity, and ligand leaching. Researchers have explored alternatives to protein A chromatography, both chromatographic and non-chromatographic, but with limited success. In this paper, we propose a non-protein A purification platform for continuous processing of monoclonal antibodies (mAbs).

Recent developments in chromatographic purification of biopharmaceuticals.

Over the last several decades, researchers have time and again proposed use of non-chromatographic methods for processing of biotherapeutic products. However, chromatography continues to be the backbone of downstream processing, particularly at process scale. There are many reasons for this, critical ones being the unparalleled scalability, robustness, and selectivity that process chromatography offers over its peers.

Author Correction: US Power Production at Risk from Water Stress in a Changing Climate.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.

US Power Production at Risk from Water Stress in a Changing Climate.

Thermoelectric power production in the United States primarily relies on wet-cooled plants, which in turn require water below prescribed design temperatures, both for cooling and operational efficiency. Thus, power production in US remains particularly vulnerable to water scarcity and rising stream temperatures under climate change and variability. Previous studies on the climate-water-energy nexus have primarily focused on mid- to end-century horizons and have not considered the full range of uncertainty in climate projections.

Integrated Chromatographic Platform for Simultaneous Separation of Charge Variants and Aggregates from Monoclonal Antibody Therapeutic Products.

Achieving consistent product quality of a biotherapeutic is a major target for any biopharmaceutical manufacturer, even more for a biosimilar producer as comparability with the innovator product is a regulatory expectation. The complexity of biotherapeutic products and their tedious manufacturing processes, however, make this a non-trivial exercise. The primary motivation of this work is to develop an integrated chromatographic platform for purification of monoclonal antibody (mAb) therapeutics that can deliver the desired separation of both charge variants and aggregates, in addition to the process related impurities like host cell proteins (HCP) and host cell DNA.

Network Science Based Quantification of Resilience Demonstrated on the Indian Railways Network.

The structure, interdependence, and fragility of systems ranging from power-grids and transportation to ecology, climate, biology and even human communities and the Internet have been examined through network science. While response to perturbations has been quantified, recovery strategies for perturbed networks have usually been either discussed conceptually or through anecdotal case studies. Here we develop a network science based quantitative framework for measuring, comparing and interpreting hazard responses as well as recovery strategies.