Skin capillary endothelial cells form a network of spatiotemporally conserved Ca activity.

Ca signaling and its regulation are important for endothelial cell (EC) functions, including local blood flow control, mechanotransduction, and barrier function. Yet the spatiotemporal organization of Ca activity and its regulation across a vascular plexus is poorly understood in an mammalian context, largely due to technical barriers. To overcome this gap in knowledge, we developed an approach to resolve Ca activity with single cell resolution in the skin vasculature of live adult mice by multi-photon imaging.

Fibroblast depletion reveals mammalian epithelial resilience across neonatal and adult stages.

Regenerative organs, like the skin, depend on niche-stem cell interactions that sustain continuous cellular turnover. In cell culture, skin fibroblasts promote epidermal stem cell proliferation and differentiation. Yet, it remains elusive how fibroblasts regulate epidermal stem cell behaviors and differentiation .