Loss of CD98HC phosphorylation by ATM impairs antiporter trafficking and drives glutamate toxicity in Ataxia telangiectasia.

Ataxia-telangiectasia is a rare genetic disorder characterized by neurological defects, immunodeficiency, cancer predisposition, radiosensitivity, decreased blood vessel integrity, and diabetes. ATM, the protein mutated in Ataxia-telangiectasia, responds to DNA damage and oxidative stress, but its functional relationship to the progressive clinical manifestation of this disorder is not understood. CD98HC chaperones cystine/glutamate and cationic/neutral amino acid antiporters to the cell membrane, and CD98HC phosphorylation by ATM accelerates membrane localization to acutely increase amino acid transport.

Anti-SARS-CoV-2 Activity of Polysaccharides Extracted from and (Rhodophyta).

Even after hundreds of clinical trials, the search for new antivirals to treat COVID-19 is still relevant. Carrageenans are seaweed sulfated polysaccharides displaying antiviral activity against a wide range of respiratory viruses. The objective of this work was to study the antiviral properties of and carrageenans against SARS-CoV-2.

Defining function of wild-type and three patient-specific mutations in a zebrafish model of embryonal rhabdomyosarcoma.

In embryonal rhabdomyosarcoma (ERMS) and generally in sarcomas, the role of wild-type and loss- or gain-of-function mutations remains largely undefined. Eliminating mutant or restoring wild-type p53 is challenging; nevertheless, understanding p53 variant effects on tumorigenesis remains central to realizing better treatment outcomes. In ERMS, >70% of patients retain wild-type , yet mutations when present are associated with worse prognosis.

Covid-19 and blooms: impacts and social issues in a mass tourism destination (Mexican Caribbean).

When the COVID-19 pandemic reached the Mexican Caribbean in late March 2020, this world-renown tourist destination had already been struggling with influxes for 5 years. The nature and magnitude of these two impacts are not directly comparable, but both have contributed to profoundly transforming the region. As extreme COVID-19 containment measures were implemented nationwide, the tourism industry contracted by 98% as over 23 million visitors failed to arrive in 2020 and 400 daily flights stopped landing at Cancun Airport.

Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Virus 1.

virus 1 (HSV-1) remains a prominent health concern widespread all over the world. The increasing genital infections by HSV-1 that might facilitate acquisition and transmission of HIV-1, the cumulative evidence that HSV-1 promotes neurodegenerative disorders, and the emergence of drug resistance signify the need for new antiviral agents. In this study, the in vitro anti-herpetic activity of sulfated polysaccharides (SPs) extracted by enzyme or hot water from seaweeds collected in France and Mexico from stranding events, were evaluated.

Trace elements in pelagic Sargassum species in the Mexican Caribbean: Identification of key variables affecting arsenic accumulation in S. fluitans.

Since 2014, the Mexican Caribbean beaches have received massive influxes of the brown seaweed Sargassum (S. fluitans III, S. natans I and S.

Species composition and chemical characterization of Sargassum influx at six different locations along the Mexican Caribbean coast.

In the last decade, unprecedented arrivals of pelagic Sargassum (Sargassum influx) have been reported for the Caribbean coasts causing severe ecological and economical affectations and remarking the necessity to characterize the phenomenon. In the north coast of the Mexican Caribbean, spatial characterization of Sargassum influx was performed in terms of its abundance, species composition and chemical content using a combination of in situ sampling and remote sensing evaluations. Sargassum influx was detected within the 25 km fringe near to Quintana Roo coast in coincidence with beach-cast events during September 2018.

Photosynthetic responses of (Chlorophyta, Bryopsidales) to interactive effects of temperature, pH, and nutrients and its carbon pathways.

In this study, we evaluated the interactive effects of temperature, pH, and nutrients on photosynthetic performance in the calcareous tropical macroalga . A significant interaction among these factors on gross photosynthesis ( ) was found. The highest values of were reached at the highest temperature, pH, and nutrient enrichment tested and similarly in the control treatment (no added nutrients) at 33 °C at the lowest pH.

Anti-Herpes simplex virus (HSV-1) activity and antioxidant capacity of carrageenan-rich enzymatic extracts from Solieria filiformis (Gigartinales, Rhodophyta).

Solieria filiformis has been reported to have molecules with various biological activities. In this study we used environmentally friendly extraction methods, such as enzyme-assisted extraction (EAE), as a first step to obtain bioactive compounds from this species. Five combinations of protease (PRO) and carbohydrase (AMG) were utilized (1:0, 0:1, 2:1, 1:1, 1:2 PRO:AMG) to obtain Water Soluble Enzymatic Hydrolysates (WSEHs).

NMR Metabolic Profiling of Sargassum Species Under Different Stabilization/Extraction Processes.

The genus Sargassum is well represented by benthic and pelagic species, some of which form massive aggregates that can travel long distances due to the force of the ocean currents. Although they constitute an essential habitat for fish and invertebrate species, large accumulations of Sargassum in coastal areas generate several economic, environmental, and health impacts. It is important to recognize the species forming these aggregates, and identify the metabolites they produce, allowing for its exploitation, and therefore, better management practices.

Short-term effects of increased CO2, nitrate and temperature on photosynthetic activity in Ulva rigida (Chlorophyta) estimated by different pulse amplitude modulated fluorometers and oxygen evolution.

Short-term effects of pCO2 (700-380 ppm; High carbon (HC) and Low carbon (LC), respectively) and nitrate content (50-5 µM; High nitrogen (HN) and Low nitrogen (LN), respectively on photosynthesis were investigated in Ulva rigida (Chlorophyta) under solar radiation (in-situ) and in the laboratory under artificial light (ex-situ). After six days of incubation at ambient temperature (AT), algae were subjected to a 4 °C temperature increase (AT+4 °C) for 3 d. Both in-situ and ex-situ maximal electron transport rate (ETRmax) and in situ gross photosynthesis (GP), measured by O2 evolution, presented highest values under HCHN, and lowest under HCLN, across all measuring systems.

Epibiont hydroids on beachcast in the Mexican Caribbean.

Massive accumulations of pelagic species of have generated recent social, economic and ecological problems along Caribbean shores. In the Mexican Caribbean, these events have prompted the study of diverse biological and ecological aspects of these macroalgae. However, studies on their associated biota, including Hydrozoa, remain scarce.

Environmentally Friendly Valorization of (Gigartinales, Rhodophyta) from IMTA Using a Biorefinery Concept.

Marine macroalgae (seaweed) are an excellent source of novel bioactive metabolites. The biorefinery concept applied to seaweed facilitates the extraction of many chemical constituents from the same biomass ensuring that the resource is used fully, generating few residues through a succession of extraction steps. In the present study, the biomass of the carragenophyte (Rhodophyta, Gigartinales) cultured in an integrated multi-trophic aquaculture (IMTA) system was evaluated to obtain valuable products by a biorefinery approach.

Synergistic Effects of Sulfated Polysaccharides from Mexican Seaweeds against Measles Virus.

Sulfated polysaccharides (SPs) extracted from five seaweed samples collected or cultivated in Mexico (Macrocystis pyrifera, Eisenia arborea, Pelvetia compressa, Ulva intestinalis, and Solieria filiformis) were tested in this study in order to evaluate their effect on measles virus in vitro. All polysaccharides showed antiviral activity (as measured by the reduction of syncytia formation) and low cytotoxicity (MTT assay) at inhibitory concentrations. SPs from Eisenia arborea and Solieria filiformis showed the highest antiviral activities (confirmed by qPCR) and were selected to determine their combined effect.

Seaweeds: an opportunity for wealth and sustainable livelihood for coastal communities.

The European, Canadian, and Latin American seaweed industries rely on the sustainable harvesting of natural resources. As several countries wish to increase their activity, the harvest should be managed according to integrated and participatory governance regimes to ensure production within a long-term perspective. Development of regulations and directives enabling the sustainable exploitation of natural resources must therefore be brought to the national and international political agenda in order to ensure environmental, social, and economic values in the coastal areas around the world.

Cytotoxic and antiproliferative constituents from Dictyota ciliolata, Padina sanctae-crucis and Turbinaria tricostata.

Context: The hexane extracts of Dictyota ciliolata Sonder ex Kützing (Dictyotaceae), Padina sanctae-crucis Børgesen (Dictyotaceae), and Turbinaria tricostata E.S. Barton (Sargassaceae) were found to exhibit cytotoxic and antiproliferative activities in vitro.

Polyamines increase carpospore output and growth during in vitro cultivation of Hydropuntia cornea.

Carpospore output and development in the marine red alga Hydropuntia cornea J. Agardh. were increased by adding polyamines (PAs) (putrescine, spermidine and spermine) singly or in combinations at 10(-9), 10(-6) and 10(-3) M.

Enhanced Antitumoral Activity of Extracts Derived from Cultured Udotea flabellum (Chlorophyta).

Very few studies have been performed to evaluate the effect of culture conditions on the production or activity of active metabolites in algae. Previous studies suggest that the synthesis of bioactive compounds is strongly influenced by irradiance level. To investigate whether the antiproliferative activity of Udotea flabellum extracts is modified after cultivation, this green alga was cultured under four photon flux densities (PFD) for 30 days.

Physicochemical properties of biodegradable polyvinyl alcohol-agar films from the red algae Hydropuntia cornea.

Agar obtained from the red alga Hydropuntia cornea was blended with polyvinyl alcohol (PVOH) in order to produce biodegradable films. In this study, we compare the properties of biopolymeric films formulated with agars extracted from H. cornea collected at different seasons (rainy and dry) in the Gulf of Mexico coast and PVOH as synthetic matrix.

Cell wall composition affects Cd2+ accumulation and intracellular thiol peptides in marine red algae.

Two red macroalgae species, Gracilaria cornea and Chondrophycus poiteaui, were evaluated for their intra and extracellular Cd2+ accumulation capacity, photosynthetic response and thiol peptide production. Algae were exposed for 3 and 7 days to 0.1 and 1 microg CdCl2 ml(-1) (0.

Physiological characterization of Dunaliella sp. (Chlorophyta, Volvocales) from Yucatan, Mexico.

Physiological responses of Dunaliella salina and Dunaliella viridis, isolated from solar saltworks on the Yucatan Peninsula, were studied. Optimal growth temperature for D. salina was 22 degrees C (3.

Regulation of two photosynthetic pigment-related genes during stress-induced pigment formation in the green alga, Dunaliella salina.

The expression of mRNAs coding for 1-deoxyxylulose-5-phosphate synthase (DXS) and phytoene synthase (PSY) were studied in Dunaliella salina grown under nitrogen-sufficient (NS) and nitrogen-limited (NL) conditions. Under NS conditions growth was 2.5 times higher than under NL conditions.