Study on non-invasive and time-course metabolomics-based biological early warning systems (BEWS) using Daphnia magna and field river water.

The application of metabolomics to the water quality monitoring system, biological early warning system (BEWS), has been proposed; however, its development has not been attempted due to challenges such as high inter-individual variability and invasive sampling requirements in metabolomics applications. In this study, we employed an extracellular metabolomics (exo-metabolomics) approach using Daphnia magna to overcome these limitations and evaluate its utility in field river water conditions. From BEWS flow-through chambers, we collected exo-metabolites under ambient, copper exposure (0-80 μg/L), and post-exposure conditions.

Comparative impacts of fragmented versus spherical microplastics on the marine rotifer Brachionus koreanus: Multigenerational chronic toxicity and multi-omics perspective.

This study investigated the toxic effects of microplastics (MPs) of different sizes and shapes on the marine rotifer, Brachionus koreanus, at both the individual and molecular levels. A multigenerational chronic toxicity test showed that only small fragmented MPs (fragment-S) significantly reduced the fecundity and lifespan of rotifers, which was enhanced in subsequent generations. Transcriptomic modulation of rotifers was most significant in the group exposed to fragment-S, followed by those exposed to large fragments (fragment-L), small beads (bead-S), and large beads (bead-L).