Frozen sperm viability and seminiferous epithelium cytology of the coati (Nasua nasua).

This study aimed to describe the cells of the seminiferous epithelium collected by fine needle aspiration cytology (FNAC), reproductive tract observations, semen collection, and sperm cryopreservation from coatis (Nasua nasua). Seven adult coatis were anesthetized, the reproductive tract was examined, and semen was collected by electroejaculation and cryopreserved using an extender based on protocol for domestic dogs, except that prior to cryopreservation, no centrifugation to remove the seminal plasma was performed. Testicular FNAC was also performed on each testis.

Assessing the sensitisation hazard of microbial pesticides: potential value of New Approach Methodologies (NAMs) to overcome current challenges.

Over the past years, the use of chemical pesticides has increased considerably worldwide, and concerns about human health and the environment have stimulated the development of safer alternatives. Biopesticides, including those with microorganisms as active substances, i.e.

Exploring the applicability of the RTgill-W1 and ZFL cell line assays in aquatic hazard assessment of mineral and organic nanomaterials.

Many fish have been used annually to evaluate chemical hazards to aquatic environments; thus, cell-based methods, such as the RTgill-W1 and ZFL cell line assays, have been proposed as alternatives to provide information on acute toxicity to fish, mostly in screening approaches and as part of a weight of evidence (WoE) approach. These methods have been developed using soluble chemicals, which can pose challenges for testing novel types of chemicals, such as nanomaterials. Cellulose nanofiber (CNF) kraft-bleached pulp, chitosan nanoparticles (CS-NP), and silica nanoparticles (SiO-NP) were evaluated regarding their toxicity to fish cells using the RTgill-W1 and ZFL cell line assays (in vitro).

Human health risk assessment for microbial pesticides in the EU: challenges and perspectives.

The risk assessment of microbial pesticides in the European Union (EU) is covered by a regulatory framework based on EU Regulation 1107/2009 and 546/2011 together with the data requirements in EU Regulation 283/2013 and 284/2013, Part B, respectively (all amended in 2022). Furthermore, several guidance documents specify the data requirements for the human health assessment. As in other regulatory contexts, the assessment of hazardous properties of a microbial plant protection product (PPP) can be based on in vivo data.

Di-n-butyl and di-iso-pentyl phthalates and their mixture increase oxidative stress and embryo-larval malformations in zebrafish (Danio rerio).

Different phthalate compounds, known as emerging contaminants, such as Di-n-butyl (DBP) and di-iso-pentyl (DiPeP) phthalates, have been found in aquatic environments and are widely used as additives to increase the flexibility of plastics. This study intended to evaluate the toxicity in zebrafish embryos and larvae of DBP and DiPeP, as well as the mixture of the two compounds. Fish embryo toxicity, neurotoxicity, oxidative stress, and genotoxicity studies were carried out in Danio rerio (zebrafish) embryos and larvae.