KCC-07, MBD2 Inhibitor, Expands the Therapeutic Window of DNA Damage Inducing Reagents in Neural Tumor Cells.

Neural tumors represent diverse malignancies with distinct molecular profiles and present particular challenges due to the blood-brain barrier, heterogeneous molecular etiology including epigenetic dysregulation, and the affected organ's critical nature. KCC-07, a selective and blood-brain barrier penetrable MBD2 (methyl CpG binding domain protein 2) inhibitor, can suppress tumor development by inducing p53 signaling, proven only in medulloblastoma. Here we demonstrate KCC-07 treatment's application to other neural tumors.

Tetrasaccharide Resin Glycosides with Nitric Oxide Production Inhibitory Activity from .

LC-HRMS/MS-based molecular-network-guided chemical investigation of led to the isolation of seven undescribed tetrasaccharide-type resin glycosides (-). Their structures were elucidated using 1D and 2D NMR and HRESIMS analysis. Isolated resin glycosides were comprised of d-glucose, d-fucose, d-quinovose, and l-rhamnose, and these monosaccharides were partially acylated with acetyl, isobutyryl, -hexanoyl, and niloyl organic acids.

Machine Learning Insights Into Social Determinants Driving Child Abuse in Pediatric Traumatic Brain Injury.

Background And Objectives: Social determinants of health (SDOH) are key drivers of health inequities, shaping disparities in patient outcomes that must be addressed. This study examines the association between SDOH and suspected child abuse (SCA) in pediatric patients sustaining traumatic brain injury (TBI), leveraging newly proposed Centers for Disease Control and Prevention (CDC)/PLACES measures to identify the most contributing measure to SCA.

Methods: A retrospective review of our institutional database (2016-2023) identified pediatric TBI cases (18 years and younger) using International Classification of Diseases, 10th Revision codes based on a modified CDC framework.

Evaluation of a Manual DNA Extraction Method Combined with In-House Aspergillus Real-Time PCR.

Background: To improve the molecular diagnostic yield for Aspergillus spp. from respiratory samples, we developed and evaluated a new DNA extraction method directly from respiratory samples combined with in-house Aspergillus real-time PCR.

Methods: We developed a method using beads and resin, where a sample is centrifuged to separate the supernatant and pellet.