Automated identification of small molecules in cryoelectron microscopy data with density- and energy-guided evaluation.

Methodological improvements in cryoelectron microscopy (cryo-EM) have made it useful in ligand-bound structure determination for biology and drug design. However, determining ligand conformation and identity is challenging at the resolutions typical for cryo-EM. Automated methods can aid in ligand conformational modeling, but current ligand identification tools-developed for X-ray crystallography data-perform poorly at resolutions common for cryo-EM.

Advancing Research and Scholarship: Report of the 2024-2025 AACP Research and Graduate Affairs Committee.

The 2024-2025 American Association of Colleges of Pharmacy (AACP) Research and Graduate Affairs Committee (or the Committee) was charged with creating an updated definition of research and scholarship for use by the Academy, performing an audit of AACP activities to identify opportunities to advance or support research and scholarship as defined by the Committee, and developing metrics for these programs. The Committee met its first charge by providing updated definitions for both research and scholarship, recognizing that each is essential to the advancement of the pharmacy profession and equally important to the Academy. To fulfil its second charge, the Committee identified opportunities to add content and objectives related to research, scholarship, and graduate education to several AACP programs.

Building Implementation Science Capacity in Academic Pharmacy: Report of the 2020-2021 AACP Research and Graduate Affairs Committee.

The 2020-2021 American Association of Colleges of Pharmacy (AACP) Research and Graduate Affairs Committee (RGAC) continued the work begun by the 2019-2020 RGAC to increase awareness of and capacity for implementation research to advance practice transformation in academic pharmacy. AACP President Anne Lin charged the RGAC with developing resources and programs for training faculty and graduate students in implementation science. The committee was further charged with developing a mechanism to pair pharmacy faculty and implementation experts on practice advancement projects.

Jurkat T-cell lines exhibit marked genomic instability affecting karyotype, mutational profile, gene expression, immunophenotype and function.

The Jurkat cell line, derived from a case of T-cell lymphoblastic leukaemia, is widely employed as a model T-cell for biomedical research, including for the preclinical characterisation of cellular therapies such as chimeric antigen receptor T-cells. Here, we characterised genomic, transcriptomic, and functional features of Jurkat clone E6-1 cells from three different laboratories and compared these with Jurkat E6-1 cells from the American Type Culture Collection (ATCC). We identified marked karyotypic heterogeneity both between and within Jurkat E6-1 populations, confirmed through chromosomal microarray.