Salmonella and antimicrobial-resistant bacteria recovered from livestock feed components include Escherichia coli ST167 expressing NDM-5 carbapenemase.

Antimicrobial-resistant bacteria, including both pathogens and commensal organisms, can be introduced into livestock populations by a variety of mechanisms including contaminated water, animal and human movement, and animal feed. We hypothesized that animal feed is an important mechanism for introduction of both Salmonella and commensal reservoir bacteria harboring mobile antimicrobial-resistance genes into livestock populations. The identification of high-risk feed components may allow targeted interventions that will reduce carriage of these organisms in food animals and ultimately improve food safety.

The genetic context of blaIMP varies among bacterial families from One Health sources.

The blaIMP resistance gene encodes a metallo-beta-lactamase in bacteria, which confers reduced susceptibility or resistance to all the beta-lactams, including carbapenems which are critical for treating life-threatening infections. The dissemination of blaIMP among various taxonomic families shows the diversity and range of horizontal gene transfer. Using short-read whole genome sequencing and bioinformatic tools, we determined the genetic motifs surrounding blaIMP present in 32 bacterial isolates recovered from environmental sources and agriculture facilities.

The impact of zinc supplementation on carbapenem MICs among bacteria expressing IMP metallo-beta-lactamase.

Antibiotic-resistant infections cause an estimated 2.8 million illnesses and 35,900 deaths annually in the USA. Carbapenems are a class of antibiotics that are generally reserved to treat life-threatening invasive infections including sepsis.

Pet treats, Salmonella, and antimicrobial resistance; a One Health problem.

Zoonotic pathogens, including Salmonella and antimicrobial resistant bacteria, may contaminate the food or treats consumed by our pets. These may directly impact the health of the pets or may be transferred to humans who are in close contact. To better understand the potential risk, we purchased 505 pet treats from pet and farm supply stores, grocery stores, and online retailers in the U.

Carbapenemase-producing Enterobacterales and their carbapenemase genes are stably recovered across the wastewater-watershed ecosystem nexus.

Carbapenemase-producing Enterobacterales (CPE) have emerged as an important nosocomial threat to hospitalized patients, but CPE can also colonize the enteric microbiota of healthy individuals in the community. We hypothesized that clinically relevant CPE are frequently transported in municipal wastewater flows to treatment plants where they are reduced but not eliminated and are subsequently discharged into nearby surface waters and disseminate in the environment. We sampled untreated influent, treated effluent, and nearby surface waters weekly for a one-year period at a single large metropolitan wastewater treatment plant (WWTP) servicing Columbus, Ohio USA.