Application of Evidence into Practice in Trauma: The Purpose to Practice (P2P) Approach to Consensus Generation.

Introduction: In trauma care, there is a need to increase communication to ensure evidence-informed, best practice care guidelines are easily accessible to all providers to yield continuity of care. Clinical guidance use is one way to address this need while employing a patient-centered team approach.

Methods: During year two of the conference series, participants gathered in person and virtually to further develop the Minimum Viable Product (MVP) created during year one.

A Better Way: Initial Acceptability Testing of Using Artificial Intelligence Tools to Accelerate Development of Trauma Clinical Guidance.

Introduction: Representatives of the trauma community have voiced a need for a new approach to developing clinical guidance. In this study, we test the initial acceptability of a proposed 12-step approach that aims to reduce the current clinical guidance timeline from more than 24 months to 24 weeks.

Methods: Investigators hypothesized that artificial intelligence (AI) tools could be leveraged to improve and make the process of clinical guidance development more efficient, facilitating AI initial output that could later be reviewed by subject matter experts (SMEs).

Transcriptomic skin niches in systemic sclerosis underpin a role for mitochondrial dysfunction.

Systemic sclerosis (SSc) is a chronic inflammatory disease characterized by fibrosis, vasculopathy and immune dysregulation. Using spatial transcriptomics on the dermis of 11 patients with SSc, we identified distinct transcriptomic niches associated with fibrosis, immune activation and mitochondrial dysfunction. Our findings highlight mitochondrial dysfunction as a central mechanism in SSc pathogenesis, ranging from adaptive remodelling in immune zones to metabolic collapse in fibrotic zones, underscoring mitochondria as a therapeutic target.

Transcriptional activation of regenerative hematopoiesis via microenvironmental sensing.

Transition between activation and quiescence states in hematopoietic stem and progenitor cells (HSPCs) is tightly governed by cell-intrinsic means and microenvironmental co-adaptation. Although this balance is fundamental for lifelong hematopoiesis and immunity, the underlying molecular mechanisms remain poorly defined. Multimodal analysis divulging differential transcriptional activity between distinct HSPC states indicates the presence of Fli-1 transcription factor binding motif in activated hematopoietic stem cells.

Single-cell atlas of human pancreatic islet and acinar endothelial cells in health and diabetes.

Characterization of the vascular heterogeneity within the pancreas has previously been lacking. Here, we develop strategies to enrich islet-specific endothelial cells (ISECs) and acinar-specific endothelial cells (ASECs) from three human pancreases and corroborate these findings with three published pancreatic datasets. Single-cell RNA sequencing reveals the unique molecular signatures of ISECs, including structural genes COL13A1, ESM1, PLVAP, UNC5B, and LAMA4, angiocrine genes KDR, THBS1, BMPs and CXCR4, and metabolic genes ACE, PASK and F2RL3.