Alterations of matrisome gene expression in multipotent mesenchymal stromal cells under physiological hypoxia in vitro.

Low O level (physiological hypoxia) is an important physical parameter in local tissue niches of multipotent mesenchymal stromal cells (MSCs). Hypoxia preconditioning is actively applied in cell therapy and regenerative medicine protocols. In the present study, the effect of physiologic hypoxia in vitro (5 % O) on the extracellular matrix of MSCs from the stromal-vascular fraction of human adipose tissue was investigated.

[THE ATOPIC DERMATITIS: HISTORY OF THEORY, PRACTICAL BASIS AND CLASSIFICATION FROM BEGINNING OF ERA TO INTERNATIONAL CLASSIFICATION OF DISEASES: PUBLICATIONS REVIEW].

The alarming trend in modern epidemiology of atopic dermatitis is omnipresent steady increase in its prevalence making the problem relevant. The purpose of the study is to shed light on historical aspects of development of ideas about atopic dermatitis and differences in classification approach. The search of publications was carried out in such Internet databases as Scopus, MedLine, еLibrary, Google Scholar according keywords atopic dermatitis, pathophysiology, history, classification.

Ex Vivo Preconditioning as a Useful Tool for Modification of the Extracellular Matrix of Multipotent Mesenchymal Stromal Cells.

Cell technologies have provided promising tools for modulating the properties of multipotent mesenchymal stem/stromal cells (MSCs) to meet the needs of cell therapy as well as tissue engineering and regenerative medicine (TERM). Ex vivo preconditioning is directed at enhancing the engraftment of MSCs and activating their secretory activity, primarily the production of soluble mediators. The present review aims to highlight the underestimated effect of the most accepted preconditioning approaches on the modification of the important set of insoluble molecules secreted by MSCs into extracellular space-the extracellular matrix (ECM).

The influence of the geroprotective cytokine on the transcriptome of young and senescent mesenchymal stem cells.

Objectives: Increasing longevity and the growing elderly population necessitate a deeper understanding of aging mechanisms to prolong productive life and improve treatments for age-related diseases linked with cellular senescence. Mesenchymal stem cells (MSCs) are crucial for maintaining tissue homeostasis, but their physiological changes during senescence are not well understood. Growth differentiation factor 11 (GDF11) has emerged as a potential rejuvenation factor, enhancing MSC viability, mobility, and angiogenic functions, which improves outcomes in ischemic models and cardiac repair.

Selection of Optimal Protocol for Decellularization of Extracellular Matrix of Mesenchymal Stromal Cells of Human Umbilical Cord Tissue.

To obtain extracellular matrix (ECM) of umbilical cord multipotent mesenchymal stromal cells (UC-MSCs), six decellularization protocols were used, based on physical (freeze/thaw) and chemical (Triton-X100, CHAPS, SDS, and NP-40) methods. The quality of decellularization was assessed using phase-contrast and fluorescence microscopy, quantitative image analysis was performed as well. It was shown that protocols based on CHAPS/NH4OH and Triton X-100/NH4OH are optimal for decellularizing UC-MSC layers and provide high-quality ECM.