Dadasnake, a Snakemake implementation of DADA2 to process amplicon sequencing data for microbial ecology.

Background: Amplicon sequencing of phylogenetic marker genes, e.g., 16S, 18S, or ITS ribosomal RNA sequences, is still the most commonly used method to determine the composition of microbial communities.

Linking Soil Fungal Generality to Tree Richness in Young Subtropical Chinese Forests.

Soil fungi are a highly diverse group of microorganisms that provide many ecosystem services. The mechanisms of soil fungal community assembly must therefore be understood to reliably predict how global changes such as climate warming and biodiversity loss will affect ecosystem functioning. To this end, we assessed fungal communities in experimental subtropical forests by pyrosequencing of the internal transcribed spacer 2 (ITS2) region, and constructed tree-fungal bipartite networks based on the co-occurrence of fungal operational taxonomic units (OTUs) and tree species.

Experimental Evidence of Functional Group-Dependent Effects of Tree Diversity on Soil Fungi in Subtropical Forests.

Deconvoluting the relative contributions made by specific biotic and abiotic drivers to soil fungal community compositions facilitates predictions about the functional responses of ecosystems to environmental changes, such as losses of plant diversity, but it is hindered by the complex interactions involved. Experimental assembly of tree species allows separation of the respective effects of plant community composition (biotic components) and soil properties (abiotic components), enabling much greater statistical power than can be achieved in observational studies. We therefore analyzed these contributions by assessing, via pyrotag sequencing of the internal transcribed spacer (ITS2) rDNA region, fungal communities in young subtropical forest plots included in a large experiment on the effects of tree species richness.

Toward a methodical framework for comprehensively assessing forest multifunctionality.

Biodiversity-ecosystem functioning (BEF) research has extended its scope from communities that are short-lived or reshape their structure annually to structurally complex forest ecosystems. The establishment of tree diversity experiments poses specific methodological challenges for assessing the multiple functions provided by forest ecosystems. In particular, methodological inconsistencies and nonstandardized protocols impede the analysis of multifunctionality within, and comparability across the increasing number of tree diversity experiments.