An ethically guided preclinical device for phenotyping H production in laboratory rodents.

Background: Dihydrogen (H) is produced endogenously by the intestinal microbiota through the fermentation of diet carbohydrates. Over the past few years, numerous studies have demonstrated the significant therapeutic potential of H in various pathophysiological contexts, making the characterization of its production in laboratory species of major preclinical importance.

Methods: This study proposes an innovative solution to accurately monitor H production in free-moving rodents while respecting animal welfare standards.

Hydrogen inhalation: in vivo rat genotoxicity tests.

Preclinical and clinical studies have shown that molecular hydrogen (H) has anti-oxidant, anti-inflammatory, and anti-apoptotic properties. Safety data are available in the literature and acute toxicity has been tested in isolated cells and laboratory animals. We have evaluates the genotoxicity of H in vivo in rats after 72 h exposure, following the International Council for Harmonization guidelines ICH S2 (R1).