Isoform-level profiling of mA epitranscriptomic signatures in human brain.

The RNA modification N6-methyladenosine (mA) is highly abundant in human brain and implicated in neurological disorders. Profiling mA within RNA isoforms is a critical step toward understanding the complex mechanisms that underpin brain function and disease; however, we lack an isoform-level atlas of mA sites in the brain. We applied Oxford Nanopore direct RNA sequencing (DRS) to three postmortem human brain regions-prefrontal cortex, caudate nucleus, and cerebellum-to simultaneously investigate the transcriptome and epitranscriptome at the isoform level.

IsoVis - a webserver for visualization and annotation of alternative RNA isoforms.

Genes commonly express multiple RNA products (RNA isoforms), which differ in exonic content and can have different functions. Making sense of the plethora of known and novel RNA isoforms being identified by transcriptomic approaches requires a user-friendly way to visualize gene isoforms and how they differ in exonic content, expression levels and potential functions. Here we introduce IsoVis, a freely available webserver that accepts user-supplied transcriptomic data and visualizes the expressed isoforms in a clear, intuitive manner.