Chromatin-associated circRNA ciCRLF3(2) regulates cell differentiation blockage via activating non-homologous end joining-based DNA repair.

DNA damage response (DDR) is a complicated network that responds to DNA lesions to prevent their accumulation; a defective DDR is one hallmark of cancer. Although targeting DDR pathways has been considered as a therapeutic approach, DDR inhibitors have also been reported ineffective for treating some low mutation burden cancers, such as Mixed-lineage leukemia (MLL)-rearranged (MLL-r) leukemia, a clinically fatal and refractory malignancy. Exploring the roles and mechanisms of DDR pathways in these low mutation burden cancers may help understand the chromatin biology and develop therapeutic strategies.

[Four new diglycosides from Momordicae Semen].

The seed kernel of Momordica cochinchinensis, i.e., Momordicae Semen, is used for medicinal purposes, but to date, no research has been reported on its chemical constituents.

A taxonomic study of the Platydracus brachycerus group with descriptions of three new species from China (Coleoptera: Staphylinidae, Staphylininae).

We here propose a species group within the genus Platydracus, the brachycerus group, that is very likely associated with termites and includes three known species: Platydracus brachycerus Smetana & Davies, 2000; Platydracus juang Smetana, 2005; and Platydracus donnyi Rougemont, 2015. We also describe three new species belonging to this group, all from China: P. smetanai sp.

C16, a novel sinomenine derivatives, promoted macrophage reprogramming toward M2-like phenotype and protected mice from endotoxemia.

Macrophage plays a critical part in host defense, tissue repair, and anti-inflammation; Macrophage reprogramming is responsible for disease development or regression. We aimed to clarify the effect of sinomenine-4-hydroxy-palmitate (C16), on macrophage reprogramming and anti-inflammatory in endotoxemia model. According to a structure modification of SIN (Sinomenine), C16 was found.

Wrist-ankle acupuncture (WAA) for precompetition nervous syndrome: study protocol for a randomized controlled trial.

Background: Precompetition nervous syndrome comprises an excessive nervous and anxiety response to the high-pressure environment preceding a sporting competition. The use of acupuncture as a treatment option for anxiety, and wrist-ankle acupuncture (WAA) specifically in this instance, has been identified as a growing trend within the Western world. In our previous study, we have confirmed the efficacy of WAA for pre-examination anxiety.

[The pathogenesis of CD4(+)T cells infiltrated into the spinal cord in rat SNL model].

Aim: To explore the infiltration pathogenesis of CD4(+);T cells following the spinal nerve ligation.

Methods: Healthy adult male SD rats were randomly divided into the spinal nerve ligation group (Tx), sham operation group (S), control group (C). the 50& mechanical paw withdrawal threshold ( 50&MWT ) was determined by up-down method; CD4(+);T cells infiltration was assessed by FACS; the mRNA levels of CCL2, CCL5 and CXCL10 were quantitated by RT-qPCR; serum cytokines were tested by ELISA kits.

[Functional study of transcription factor Hlx modified dendritic cell line DC2.4].

Aim: To transfect Hlx into mouse dendritic cell line DC2.4 and observe the effect of hlx on function of dendritic cells.

Methods: The eukaryotic expression vector PIRES2-EGFP/Hlx was transfected into DC2.

[The expression, identification of human HMGB1 B box and preparation of monoclonal antibodies against HMGB1 B box].

Aim: To express human HMGB1 B box protein and obtain monoclonal antibodies (mAbs) against HMGB1 B box for further study of the function of human HMGB1 protein.

Methods: pET28-HMGB1 B box plasmid transfected the DH5α, then expressed. And the extracted protein was purified by protein purification system.

Diacetonitrile-(3-{2-[8-(2-bromo-eth-oxy)-9,10-dioxoanthracen-1-yl-oxy]eth-yl}-1-(2-pyridyl-meth-yl)imidazolium)silver(I) bis-(hexa-fluorido-phosphate).

The title compound, [Ag(C(27)H(23)BrN(3)O(4))(CH(3)CN)(2)](PF(6))(2), is a mononuclear salt species in which the silver(I) atom is coordinated by one ligand and two acetonitrile mol-ecules and exhibits a distorted T-shaped coordination. The asymmetric unit contains one independent cation and two independent hexa-fluorido-phosphate anions, one of which is disordered over two positions in a 0.756 (11):0.

[Effect of total saponins of Rubus parviflolius (TSRP) on change of hydrated amount and blood-brain barrier in rats during focal cerebral ischemic/reperfusion].

Objective: To explore the effects of total saponins of Rubus parviflolius (TSRP) on brain edema and blood brain barrier in rats.

Method: The model of local cerebral ischemia was established in rats by reversible inserting a nylon thread into the anterior cerebral artery through the internal carotid artery brain hydrated amount and content change of Evan' s blue (EB) in cortex subjected to 2h middle cererbral artery occlusion (MACO) followed by 6 h, 24 h, 48 h, 72 h reperfusion and effect of TSRP. penetrability of blood brain-barrier (BBB) the index includes brain hydrated amount and penetrability of blood brain-barrier BBB.