ACS Appl Mater Interfaces
April 2025
Optical phase imaging has become a pivotal tool in biomedical research, enabling label-free visualization of transparent specimens. Traditional optical phase imaging techniques, such as Zernike phase contrast and differential interference contrast microscopy, fall short of providing quantitative phase information. Digital holographic microscopy (DHM) addresses this limitation by offering precise phase measurements; however, off-axis configurations, particularly Mach-Zehnder and Michelson-based setups, are often hindered by environmental susceptibility and bulky optical components due to their separate reference and object beam paths.
View Article and Find Full Text PDFThis paper proposes an alternative method for grating period measurement based on heterodyne grating interferometry. The optical configurations for measuring the period of reflection/transmission gratings were demonstrated, and four commercially available gratings were used to evaluate the effectiveness of the proposed method. Based on the phase-lock technique, the grating period could be obtained immediately through the phase wrapped/unwrapped process.
View Article and Find Full Text PDFDigital holographic microscopy (DHM) is a powerful quantitative phase imaging (QPI) technique that is capable of recording sample's phase information to enhance image contrast. In off-axis DHM, high-quality QPI images can be generated within a single recorded hologram, and the system stability can be enhanced by common-path configuration. Diffraction gratings are widely used components in common-path DHM systems; however, the presence of multiple diffraction beams leads to system power loss.
View Article and Find Full Text PDFPhase contrast imaging techniques enable the visualization of disparities in the refractive index among various materials. However, these techniques usually come with a cost: the need for bulky, inflexible, and complicated configurations. Here, we propose and experimentally demonstrate an ultracompact meta-microscope, a novel imaging platform designed to accomplish both optical and digital phase contrast imaging.
View Article and Find Full Text PDFRecently, an optical scanning holographic system with a polarization directed flat lens was proposed to realize coaxial scanning holography (CSH). The advantage of CSH is its small form factor and the stability. However, the diffraction efficiency of the polarization directed flat lens cannot be 100%, and thus there is always zeroth order light in the scanning beam.
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