Publications by authors named "Chalapathi Pradeep"
Unlabelled: The Fall armyworm, , is a globally important invasive pest, primarily on corn, causing severe yield loss. Overuse of synthetic chemicals has caused significant ecological harm, and in many instances control has failed. Therefore, developing efficient, environmentally friendly substitutes for sustainable management of this pest is of high priority.
View Article and Find Full Text PDFEmbryonic microinjection of ribonucleoprotein complex (Cas9+sgRNA) of white gene in melon fly, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) produced white eye phenotype.
Arch Insect Biochem Physiol
December 2023
Article Synopsis
- The melon fly (Zeugodacus cucurbitae) is a significant pest affecting cucurbit crops, leading to considerable yield loss and economic impact.
- The CRISPR/Cas9 system is a powerful genome editing tool that can target specific genes to create stable genetic changes, which can help manage pest traits more efficiently without needing extensive genetic data.
- In this study, researchers successfully used CRISPR/Cas9 to mutate the white gene in melon flies, resulting in a white eye phenotype, which aids in understanding gene function and could lead to innovative pest control methods like precision guided sterile insect technique (pgSIT).
CRISPR/Cas9 mediated mutagenesis of the major sex pheromone gene, acyl-CoA delta-9 desaturase (DES9) in Fall armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae).
Int J Biol Macromol
December 2023
The Fall armyworm, Spodoptera frugiperda is a significant global pest causing serious yield loss on several staple crops. In this regard, this pest defies several management approaches based on chemicals, Bt transgenics etc., requiring effective alternatives.
View Article and Find Full Text PDFCRISPR/Cas9-mediated mutagenesis of Sex lethal (Sxl) gene impacts fertility of the Fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae).
Arch Insect Biochem Physiol
October 2023
Identification of novel approaches for managing the global pest, the Fall armyworm, Spodoptera frugiperda, is the need of the hour, as it defies many management strategies including synthetic chemicals, Bt transgenics, and so on. Recently CRISPR/Cas9-based genome editing opened up newer avenues to design novel pest management strategies such as precision-guided sterile insect technique (pgSIT). In this regard, genes governing sex determination, egg reproduction, and spermatogenesis could be the prime targets for genome editing.
View Article and Find Full Text PDFFirst report on the utility of pupal case for early determination of CRISPR/Cas9 ribonucleoprotein mediated genomic edits in the oriental fruit fly, Bactrocera dorsalis (Hendel) (Tephritidae: Diptera).
Arch Insect Biochem Physiol
August 2023
Article Synopsis
- The Oriental fruit fly, a major pest, disrupts global fruit trade and requires effective management methods, with sterile insect technique (SIT) being a leading chemical-free option.
- The rising use of CRISPR/Cas9 for precise genetic editing in pest control aims to create mutations without compromising the fitness of the fruit flies.
- This study demonstrates a method to identify successful genomic edits in B. dorsalis through analysis of shed pupal cases, allowing for easier selection of edited individuals while bypassing the need to wait for adult life cycles.