Assessment of Diagnostic Value of Post Mortem Tongue Tip Fluids for Disease Detection in Growing Pigs.

Assessing whether post mortem samples can provide value for monitoring during the post-weaning stages is necessary as this methodology can be adopted by the industry when resources are scarce. The objectives of this study are to assess pathogen detection in tongue tip fluids (TTFs), oronasal swabs (ONSs), rectal swabs (RSs), and superficial inguinal lymph nodes (SILNs). Two farms (one wean-to-finish farm and one finishing farm) undergoing a porcine reproductive and respiratory syndrome virus (PRRSV) outbreak were included, and 30 dead pigs were sampled during each farm visit.

Experimental evidence of vaccine-driven evolution of respiratory syndrome virus type 2.

Despite extensive use of vaccination, porcine reproductive and respiratory syndrome virus type 2 (PRRSV-2) continues to evolve, likely driven by escape from natural or vaccine-derived immunity. However, direct evidence of vaccine-induced evolutionary pressure remains limited. Here, we tracked the evolution of PRRSV-2 sublineage 1A strain IA/2014 (variant 1A-unclassified) genome from infection chains of sequentially infected pigs under different immune conditions.

Mitigating between-farm disease transmission through simulating vehicle rerouting and enhanced cleaning and disinfection protocols.

The role of contaminated vehicles and the effectiveness of preventive strategies, such as cleaning and disinfection (C&D), in spreading infectious diseases among commercial swine farms under field conditions remains largely unexplored. This study aimed to reduce the risk of between-farm disease transmission through vehicle contacts by rerouting vehicles while considering C&D events and effectiveness. Vehicles were ranked based on specific criteria, including disease status of visited farms, vehicle contact network communities, C&D events, and shipment time efficiency.

Economic impact of productivity losses attributable to porcine reproductive and respiratory syndrome virus in United States pork production, 2016-2020.

The porcine reproductive and respiratory syndrome virus (PRRSV) continues to pose a major economic burden on the swine industry in the United States (US). This study estimates the annual cost of PRRSV-related productivity losses using US data from 2016 to 2020. Productivity data were collected from commercial swine herds with known PRRSV, porcine epidemic diarrhea virus (PEDV), and Mycoplasma hyopneumoniae statuses.

Evaluation of the infectivity of three porcine reproductive and respiratory syndrome virus (PRRSV) variants.

Porcine reproductive and respiratory syndrome virus (PRRSV) continues to burden the US swine industry significantly. In some instances, the virus evaded biosecurity measures, remaining viable in sufficient concentrations to cause an outbreak. Little is known about differences in infectivity among viral variants.

Complete Genome and Recombination Analysis of a Novel Porcine Reproductive and Respiratory Syndrome Virus 2 (Variant 1H.18) Identified in the Midwestern USA.

Porcine Reproductive and Respiratory Syndrome Virus 2 (PRRSV-2) represents one of the greatest threats to global pork production. Increased incidence of a genetic variant of Porcine Reproductive and Respiratory Syndrome Virus (variant 1H.18) was recently reported in the Midwestern USA Sequence comparisons in the ORF5 region indicate that 1H.

Senecavirus A Incidence in U.S. Breeding Herds: A Decade of Surveillance Data.

Senecavirus A (SVA) is an endemic pathogen with ongoing relevance to the U.S. swine industry due to its clinical resemblance to foot-and-mouth disease (FMD) and its potential impact on animal health and trade.

Current Evolutionary Dynamics of Porcine Epidemic Diarrhea Virus (PEDV) in the U.S. a Decade After Introduction.

Porcine Epidemic Diarrhea Virus (PEDV) was introduced in the United States (U.S.) in 2013, spreading rapidly and leading to economic losses.

In vitro evaluation of porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 sequences in samples containing PRRSV modified-live vaccine and wild-type strains.

Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses and is a major challenge to the swine industry. The PRRS virus (PRRSV) has high rates of mutation and evolution. We examined the influence on ORF5 Sanger sequencing outcomes of various concentrations of a wild-type (WT) PRRSV (lineage 1A RFLP 1-7-4) and a modified-live vaccine (MLV) virus (lineage 5 RFLP 2-5-2) in the same sample.

Simulation of Premovement Active Surveillance Protocols for Moving Finishing Pigs to a Harvest Facility from a Control Area during an Outbreak of African Swine Fever in the United States.

Movement restrictions are a critical component of response plans for an African swine fever (ASF) outbreak in the United States. These restrictions are likely to include requiring permits to move animals and products within, into, and out of 5-km control areas (CAs) established around confirmed positive farms. For quarantined finishing farms located within a CA, diagnostic testing is an expected criterion for receival of a permit to move pigs to a harvest facility or removal of quarantine.

Postepidemic Epidemiology of Porcine Epidemic Diarrhea Virus in the United States.

Porcine epidemic diarrhea virus (PEDV) emerged in the United States (U.S.) swine population in 2013, initiating an initial significant epidemic followed by a state of endemicity in the U.

Truck cleaning and disinfection, and the risk of PRRSV dissemination in multi-site pig production systems in the United States: A network-epidemiological model approach.

Porcine reproductive and respiratory syndrome virus (PRRSV) poses a significant threat to pig health, particularly in multi-site industrial pig farming systems. These systems, which involve raising pigs at different locations based on their age and transporting them via trucks and trailers, may increase the risk of pathogen transmission through contaminated vehicles. Given the importance of preventing PRRSV infection, vehicle cleaning and disinfection (C&D) are crucial for disease control.

Stability of five strains of PRRSV in tap water at different temperatures.

Porcine reproductive and respiratory syndrome (PRRS) is considered one of the most economically important diseases of swine, as outbreaks in the U.S. continue occurring despite current rigorous biosecurity measures.

Differential shaping of T cell responses elicited by heterologous ChAd68/self-amplifying mRNA SIV vaccine in macaques in combination with αCTLA4, αPD-1, or FLT3R agonist.

While therapeutic vaccines are a promising strategy for inducing human immunodeficiency virus (HIV) control, HIV vaccines tested to date have offered limited benefit to people living with HIV. The barriers to success may include the use of vaccine platforms and/or immunogens that drive weak or suboptimal immune responses, immune escape and/or immune dysfunction associated with chronic infection despite effective antiretroviral therapy. Combining vaccines with immune modulators in a safe manner may address some of the challenges and thus increase the efficacy of therapeutic HIV vaccines.

Comparative Adsorption of Porcine Reproductive and Respiratory Syndrome Virus Strains to Minnesota Soils.

Porcine reproductive and respiratory syndrome (PRRS) is an endemic disease affecting the swine industry. The disease is caused by the PRRS virus (PRRSV). Despite extensive biosecurity and control measures, the persistence and seasonality of the virus have raised questions about the virus's environmental dynamics during the fall season when the yearly epidemic onset begins and when crop harvesting and manure incorporation into the field occur.

PRRSV-2 variant classification: a dynamic nomenclature for enhanced monitoring and surveillance.

Existing genetic classification systems for porcine reproductive and respiratory syndrome virus type 2 (PRRSV-2), such as restriction fragment length polymorphisms and sub-lineages, are unreliable indicators of close genetic relatedness or lack sufficient resolution for epidemiological monitoring routinely conducted by veterinarians. Here, we outline a fine-scale classification system for PRRSV-2 genetic variants in the United States. Based on >25,000 U.

Weathering the storm: Extreme weather events and their association with PED and PRRS occurrence.

Porcine epidemic diarrhea (PED) and Porcine reproductive and respiratory syndrome (PRRS) are viral diseases that continue to challenge the US swine industry. Despite many known risk factors, unusual circumstances associated with their occurrence continues to be poorly explained. We investigated if extreme weather events (flood, heavy rain, high wind and tornadoes, measured at a county-level) are associated with the occurrence of both diseases up to ten weeks after the occurrence of the weather event using a case control study and logistic regression modeling to control for covariates.

Porcine reproductive and respiratory syndrome virus variant emergence: Example and considerations for prospective monitoring.

New PRRSV variants are constantly emerging due to the rapid evolution of this virus. We aimed to describe the emergence of a new PRRSV variant within sub-lineage 1 C, its space-time distribution, and its impact on affected herds. Additionally, we discuss considerations on how to monitor emerging PRRSV variants.

Assessment of porcine reproductive and respiratory syndrome virus (PRRSV) farm surface contamination through environmental sampling.

Background: During the fall of 2020, the porcine reproductive and respiratory syndrome virus (PRRSV) L1C.5 variant emerged and rapidly spread throughout southern Minnesota generating questions regarding possible transmission routes. This study aimed to investigate whether PRRSV could be detected on surfaces inside and outside pig barns housing L1C.

Further Evidence That Science-Based Biosecurity Provides Sustainable Prevention of Porcine Reproductive and Respiratory Syndrome Virus Infection and Improved Productivity in Swine Breeding Herds.

Porcine reproductive and respiratory syndrome virus (PRRSV) is a globally significant pathogen of pigs. Preventing the entry of PRRSV into swine breeding herds enhances animal health and welfare. A recently published retrospective cohort study reported significant differences in PRRSV incidence risk between breeding herds that practiced Next Generation Biosecurity (NGB) COMPLETE, versus herds that practiced a partial approach (NGB INCOMPLETE) over a 2-year period.

Postmortem Sampling in Piglet Populations: Unveiling Specimens Accuracy for Porcine Reproductive and Respiratory Syndrome Detection.

Specimens collected from dead pigs are a welfare-friendly and cost-effective active surveillance. This study aimed to evaluate the accuracy of different postmortem specimens from dead piglets for disease detection, using PRRSV as an example. Three farrow-to-wean farms undergoing PRRSV elimination were conveniently selected.

Enhancing disease surveillance and preparedness: An early warning tool for disease occurrence in U.S. swine breeding herds.

Understanding regional disease risk is critical for swine disease prevention and control. Since 2011, the Morrison Swine Health Monitoring Project (MSHMP) has strengthened partnerships among practitioners and producers to report health events (e.g.

Porcine Deltacoronavirus Occurrence in the United States Breeding Herds since Its Emergence in 2014.

PDCoV, an enveloped RNA virus, causes atrophic enteritis in neonatal piglets, leading to diarrhea, malabsorption, dehydration, and death. The study aims to fill the gap in the current epidemiological information about PDCoV in the U.S.

Predicting Potential PRRSV-2 Variant Emergence through Phylogenetic Inference.

Porcine reproductive and respiratory syndrome (PRRS) is a significant pig disease causing substantial annual losses exceeding half a billion dollars to the United States pork industry. The cocirculation and emergence of genetically distinct PRRSV-2 viruses hinder PRRS control, especially vaccine development. Similar to other viral infections like seasonal flu and SARS-CoV-2, predictive tools for identifying potential emerging viral variants may prospectively aid in preemptive disease mitigation.

Survival of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in the Environment.

Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important diseases of swine, with losses due to poor reproductive performance and high piglet and growing pig mortality. Transmission of porcine reproductive and respiratory syndrome virus (PRRSV) may occur by both direct and indirect routes; the latter includes exposure to PRRSV-contaminated fomites, aerosols, and arthropod vectors. This review has collected available data on the ex-vivo environmental stability and persistence of PRRSV in an effort to highlight important sources of the virus and to determine the role of environmental conditions on the stability of the virus, especially temperature.