Publications by authors named "Celine Meyrignac"
Development of 3D gingival in vitro models using primary gingival cells.
In Vitro Cell Dev Biol Anim
September 2024
Article Synopsis
- Since March 2013, Europe has banned animal testing for cosmetic ingredient toxicity, applying to all personal care products.
- Researchers are looking to create an "in-house model" to better understand oral diseases and evaluate cosmetic ingredients' toxicity and compatibility.
- Their expertise in tissue engineering led to successful reconstruction of human oral tissues from gingival cells, mimicking natural tissue characteristics for research purposes.
Article Synopsis
- Air pollution, specifically ultrafine particles (UFP), is linked to increased skin aging and affects the skin's ability to renew itself.
- Exposure to UFP leads to the generation of reactive oxygen species and disrupts important markers in keratinocyte stem cells (KSC).
- The study suggests that UFP pollution poses significant risks to skin health, complicating the skin's protective functions and accelerating aging processes.
Reconstructed human epidermis equivalents (RHE) have been developed as a clinical skin substitute and as the replacement for animal testing in both research and industry. KiPS, or keratinocytes derived from induced pluripotent stem cells (iPSCs) are frequently used to generate RHE. In this study, we focus on the mitochondrial performance of the KiPS derived from iPSCs obtained from two donors.
View Article and Find Full Text PDFSince 30 years, bioengineering allowed to reconstruct human tissues using normal human cells. Skin is one of the first organ to be reconstructed thanks to the development of specific cell culture media and supports favoring the culture of human skin cells, such as fibroblasts, keratinocytes, or melanocytes. Skin models have evolved from epidermis to complex models including a dermis.
View Article and Find Full Text PDFEstablishment and performance assessment of an in-house 3D Reconstructed Human Cornea-Like Epithelium (RhCE) as a screening tool for the identification of liquid chemicals with potential eye hazard.
Toxicol In Vitro
December 2019
Article Synopsis
- The project aimed to create a 3D reconstructed human cornea-like epithelium (RhCE) for early-stage product screening of liquid chemicals regarding eye safety.
- Histological analyses showed that the RhCE model, made with Normal Human Keratinocytes, has a well-organized structure and effective barrier functionality, making it suitable for testing.
- The model demonstrated high prediction accuracy (84.4%) and reliability in classifying chemicals based on their eye damage potential, indicating it could serve as a useful tool for eye hazard identification.
In recent years, in vitro skin models combining cell biology and tissue engineering have been developed in order to replace animal models for toxicological studies and to serve as research support to better understand skin biology. This study reports the development and characterization of a epidermal tissue equivalent meant to be used to develop and to evaluate the effect of applied cosmetic ingredients, and for alternative toxicological testing. This epidermis equivalent model was characterized relative to the morphological characteristics of short- and long-term maintained tissues by performing histological studies.
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