Publications by authors named "Casper Normann Nurup"
Recombinant production of membrane proteins in yeast.
Methods Enzymol
March 2022
Biochemical pathways are compartmentalized in living cells. This permits each cell to maintain chemical compositions that differ between the cytosol, intracellular organelles and the external environment. Achieving this requires each compartment to be very selective in what is allowed to enter and leave.
View Article and Find Full Text PDFas a superior host for overproduction of prokaryotic integral membrane proteins.
Curr Res Struct Biol
February 2021
Article Synopsis
- * A study compared the expression of five different families of prokaryotic IMPs in yeast and found that yeast outperformed other methods for four IMPs, even successfully expressing zinc transporters that were previously undetectable.
- * The research highlights the importance of the fusion tag's localization for improving protein yield and quality, providing a comprehensive strategy for optimizing the production of prokaryotic IMPs in yeast for further research and characterization.
The sparse number of high-resolution human membrane protein structures severely restricts our comprehension of molecular physiology and ability to exploit rational drug design. In the search for a standardized, cheap and easily handled human membrane protein production platform, we thoroughly investigated the capacity of S. cerevisiae to deliver high yields of prime quality human AQPs, focusing on poorly characterized members including some previously shown to be difficult to isolate.
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