Integrating kinetic models, gene circuits, and biofilm dynamics for enhanced exopolysaccharide production in nitrifying bacterial consortia.

Bacterial consortia enriched from domestic wastewater were studied through kinetic and genetic circuit modelling to optimize extracellular polysaccharide (EPS) production and nitrogen removal. This study integrates kinetic modelling and synthetic biology to optimize consortia performance. Growth kinetics were simulated using extended Monod and Verhulst models, under controlled nitrogen flux (10 ppm NH₄Cl), yielding a maximum biomass concentration (OD₅₉₀ = 5.