Impact of tebipenem pivoxil on the intestinal microbiota and on establishment of colonization with carbapenem-resistant in mice.

Tebipenem pivoxil has potent activity against pathogens, but requires combination with β-lactamase inhibitor to achieve activity against carbapenemase producers, including metallo-β-lactamases (MBLs). Herein, we evaluate the potential of tebipenem pivoxil, alone and in combination with the prodrug of the experimental MBL inhibitor CS319 (CS319-piv-SAc), to disrupt the indigenous mice microbiota of the colon and promote colonization by pathogens. The effect of antibiotic treatment (daily for 3 days with subcutaneous saline [control], subcutaneous clindamycin, oral tebipenem pivoxil alone and in combination with CS319-piv-Sac, or oral CS319-piv-Sac) on the intestinal microbiota was assessed by culture for enterococci and facultative Gram-negative bacilli and by 16S rRNA amplicon sequencing.

Pulsed dosing and extended daily dosing of oral vancomycin do not facilitate clearance of colonization in mice.

Vancomycin taper and pulse regimens are commonly used to treat recurrent infections, but the mechanism by which these regimens might reduce recurrences is unclear. Here, we used a mouse model to test the hypothesis that pulse dosing of vancomycin after a 10-day treatment course enhances clearance of from the intestinal tract. Mice with colonization received 10 days of once-daily oral vancomycin followed by 20 days of treatment with saline (controls), daily vancomycin, or pulse dosing of vancomycin every 2 or 3 days.

Efficacy of 23 commonly used liquid disinfectants against isolates from the 4 major clades.

We tested the effectiveness of 23 disinfectants used in healthcare facilities against isolates from the 4 major clades of Sporicidal disinfectants were consistently effective, whereas quaternary-ammonium disinfectants had limited activity. Quaternary-ammonium-alcohol and hydrogen-peroxide-based disinfectants varied in effectiveness against .

Effectiveness of a novel 1-step cleaner and disinfectant against .

A novel 1-step anionic surfactant disinfectant was effective against isolates from the 4 major phylogenetic clades as well as methicillin-resistant (MRSA) and the enveloped virus bacteriophage Phi6. This anionic surfactant disinfectant may be a useful addition to the disinfectant products available for use against .

Effectiveness and real-world materials compatibility of a novel hydrogen peroxide disinfectant cleaner.

A novel 4% hydrogen peroxide disinfectant was effective against methicillin-resistant Staphylococcus aureus (MRSA), Clostridioides difficile spores, carbapenem-resistant Escherichia coli, and 2 strains of Candida auris. In laboratory testing, a sodium hypochlorite disinfectant caused fading and loss of pliability of a hospital mattress, but the hydrogen peroxide disinfectant did not. These findings suggest that the hydrogen peroxide-based disinfectant may be a useful addition to the sporicidal disinfectant products available for use in healthcare settings.

Microbial bioburden of inpatient and outpatient areas beyond patient hospital rooms.

Objective: To investigate the frequency of environmental contamination in hospital areas outside patient rooms and in outpatient healthcare facilities.

Design: Culture survey.

Setting: This study was conducted across 4 hospitals, 4 outpatient clinics, and 1 surgery center.

Evaluation of 2 Ultraviolet-C Light Boxes for Decontamination of N95 Respirators.

Background: Ultraviolet-C (UV-C) light devices are effective in reducing contamination on N95 filtering facepiece respirators. However, limited information is available on whether UV-C devices meet the Food and Drug Administration's (FDA) microbiological requirements for Emergency Use Authorization (EUA) for respirator bioburden reduction.

Methods: We tested the ability of 2 UV-C light boxes to achieve the 3-log microorganism reductions required for EUA for reuse by single users.

Evaluation of a continuously active disinfectant for decontamination of portable medical equipment.

A single spray application of a continuously active disinfectant on portable equipment resulted in significant reductions in aerobic colony counts over 7 days and in recovery of Staphylococcus aureus and enterococci: 3 of 93 cultures (3%) versus 11 of 97 (11%) and 20 of 97 (21%) in quaternary ammonium disinfectant and untreated control groups, respectively.

Efficacy of relatively low-cost ultraviolet-C light devices against .

Background: Ultraviolet-C (UV-C) light devices could be useful to reduce environmental contamination with . However, variable susceptibility of strains to UV-C has been reported, and the high cost of many devices limits their use in resource-limited settings.

Objective: To evaluate the efficacy of relatively low-cost (<$15,000 purchase price) UV-C devices against strains from the 4 major phylogenetic clades.

Ultraviolet-C (UV-C) monitoring made simple: Colorimetric indicators to assess delivery of UV-C light by room decontamination devices.

Objective: To evaluate the use of colorimetric indicators for monitoring ultraviolet-C (UV-C) light delivery to sites in patient rooms.

Methods: In laboratory testing, we examined the correlation between changes in color of 2 commercial colorimetric indicators and log10 reductions in methicillin-resistant Staphylococcus aureus (MRSA) and Clostridioides difficile spores with exposure to increasing doses of UV-C from a low-pressure mercury room decontamination device. In patient rooms, 1 of the colorimetric indicators was used to assess UV-C dose delivery to 27 sites in the room.

Timing and route of contamination of hospitalized patient rooms with healthcare-associated pathogens.

Objective: To investigate the timing and routes of contamination of the rooms of patients newly admitted to the hospital.

Design: Observational cohort study and simulations of pathogen transfer.

Setting: A Veterans' Affairs hospital.