Optimized formulation of a three-component extract mixture from Moroccan Crocus sativus L. (Stigmas, leaves, and Tepals) for enhanced antioxidant activity.

This study evaluated the antioxidant potential of a three-component mixture derived from Crocus sativus L. stigmas, leaves, and tepals, aiming to valorize these underutilized by-products. Hydroethanolic extracts were chemically profiled using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD), identifying major bioactive compounds including crocin (11.

Mining the whole genome sequence of Streptomyces cyaneofuscatus strain CTM50504 isolated from the Aïn El-Atrous hot spring, Tunisia, for the discovery of extremozymes: Promising properties of protease activity.

Next-generation sequencing (NGS) methods allow for the generation of data leading to a greater understanding of the potential functionality and dynamics of microorganisms within their biotopes. The enormous volume of data that NGS produces necessitates understanding structural and functional genomics through the application of various omics techniques. Streptomyces cyaneofuscatus CTM50504 is a potential extracellular hydrolase producer isolated from a terrestrial hot spring, Aïn El-Atrous, Korbous (Nabeul, Tunisia).

Optimization of antibacterial and antifungal activities in Moroccan saffron by-products using mixture design and simplex centroid methodology.

The health risks associated with synthetic preservatives have intensified the search for natural antimicrobial alternatives. Crocus sativus L. (saffron) generates abundant by-products, such as tepals and leaves, which are rich in bioactive compounds with demonstrated antimicrobial potential.

Purification and Biochemical Characterization of a New Thermostable and Detergent-Stable Serine Protease From a Novel Thermo-Halotolerant Bacterial Strain, Laceyella sacchari Strain BK-TM.

Laceyella sacchari strain BK-TM, isolated from the Salt Lake of Chott Ech Chergui (El-Bayadh, Algeria), was found to produce an extracellular thermostable serine protease (SPLS). The highest level of protease activity detected after 6 days of incubation at 50°C was 44,600 U/mL. SPLS was purified after heat treatment for 5 min at 80°C, subjected to ammonium sulfate fractionation (80%), and Sephacryl S-200 High Resolution (HR) column purification.

Advanced Zymographic Techniques for Detection and In-Depth Characterization of Microbial Proteases in Enzymology Research.

Proteases or peptidases are hydrolases that break down polypeptide chains into smaller peptide subunits or amino acids. The search for proteases is important and often required using the sensitive screening technique known as zymography. Zymography is a two-stage technique that involves separating proteins, via electrophoresis, followed by the detection of protease activity.

Bioinformatics analyses of lignin peroxidases from the smoky bracket fungi for endocrine disrupting chemical bioremediation.

Lignin peroxidases (LiP; EC 1.11.1.

Preparation and Biochemical Characterization of Thom P22 Lipase Immobilization Using Adsorption, Encapsulation, and Adsorption-Encapsulation Approaches.

This work describes the immobilization and the characterization of purified Thom P22 lipase (PCrL) using adsorption, encapsulation, and adsorption-encapsulation approaches. The maximum activity of the immobilized PCrL on CaCO microspheres and sodium alginate beads was shifted from 37 to 45 °C, compared with that of the free enzyme. When sodium alginate was coupled with zeolite or chitosan, the immobilization yield reached 100% and the immobilized PCrL showed improved stability over a wide temperature range, retaining all of its initial activity after a one-hour incubation at 60 °C.

Comprehensive analysis of bioactive compounds in stamens extracts by HPLC-DAD: investigating antidiabetic activity via , , and molecular docking simulation.

Medicinal herbs used in traditional diabetes treatment are a rich source of anti-diabetic compounds. Pancreatic α-amylase inhibitors offer an effective strategy to reduce postprandial hyperglycemic levels via control of starch degradation. In this context, our study for the first time investigates the effect of stamens extracts on α-amylase inhibition.

Production of a new chitinase from Nocardiopsis halophila TN-X8 utilizing bio-waste from the blue swimming crab: enzyme characterization and immobilization.

In accordance with the framework of the Circular Blue Bioeconomy in the Mediterranean region, the objective of this study was to evaluate the biotransformation of blue swimming crab (Portunus segnis) residues obtained from the port of Sfax by an extracellular chitinase produced by Nocardiopsis halophila strain TN-X8 isolated from Chott El Jerid (Tozeur, Tunisia). From the analysis of multiple extremophilic Actinomycetota, it was determined that strain TN-X8 exclusively utilized 60 g/L of raw blue swimming crab as its carbon and energy source, achieving a chitinase activity of approximately 950 U/mL following a 6-day incubation period at 40 °C. Pure chitinase, designated as ChiA-Nh30, was obtained after heat treatment, followed by ammonium sulfate fractionation and Sephacryl® S-200 column chromatography.

Biochemical characterization of an alkaline and detergent-stable Lipase from Fusarium annulatum Bugnicourt strain CBS associated with olive tree dieback.

This work describes a novel extracellular lipolytic carboxylester hydrolase named FAL, with lipase and phospholipase A1 (PLA1) activity, from a newly isolated filamentous fungus Ascomycota CBS strain, identified as Fusarium annulatum Bunigcourt. FAL was purified to about 62-fold using ammonium sulphate precipitation, Superdex® 200 Increase gel filtration and Q-Sepharose Fast Flow columns, with a total yield of 21%. The specific activity of FAL was found to be 3500 U/mg at pH 9 and 40°C and 5000 U/mg at pH 11 and 45°C, on emulsions of triocanoin and egg yolk phosphatidylcholine, respectively.

Antifungal activity of lactic acid bacteria and their application in food biopreservation.

Lactic acid bacteria (LAB) are ubiquitous bacteria associated with spontaneous lactic fermentation of vegetables, dairy and meat products. They are generally recognized as safe (GRAS), and they are involved in transformation of probiotic lacto-fermented foods, highly desired for their nutraceutical properties. The antifungal activity is one of the exciting properties of LAB, because of its possible application in food bio-preservation, as alternative to chemical preservatives.

Preparation, characterization, immobilization, and molecular docking analysis of a novel detergent-stable subtilisin-like serine protease from Streptomyces mutabilis strain TN-X30.

We recently described the production of a detergent-biocompatible crude protease from Streptomyces mutabilis strain TN-X30. Here, we describe the purification, characterization, and immobilization of the serine alkaline protease (named SPSM), as well as the cloning, sequencing, and over-expression of its corresponding gene (spSM). Pure enzyme was obtained after ammonium sulphate precipitation followed by heat-treatment and Sephacryl® S-200 column purification.

Purification, Biochemical and Kinetic Characterization of a Novel Alkaline -1,3-Regioselective Triacylglycerol Lipase from Thom Strain P22 Isolated from Moroccan Olive Mill Wastewater.

A novel extracellular lipase from a filamentous fungus Ascomycota strain, P22, was isolated from olive mill wastewater, then purified and characterized. This strain was identified as Thom based on sequencing analyses. Thom strain P22 lipase (PCrL) was purified 63-fold to homogeneity using ammonium sulfate precipitation and chromatography on a Q-Sepharose Fast Flow column, with a total yield of 34%.

Protective effects of dietary Kefir against aflatoxin B1-induced hepatotoxicity in Nile tilapia fish, .

The effect of dietary Kefir supplementation on the biometric, biochemical, and histological parameters of Nile tilapia () exposed to aflatoxin B1 (AFB1, 200 µg/kg diet) contamination was studied. The yeasts were dominant in Kefir followed by lactic and acetic acid bacteria. The Kefir showed relatively interesting antioxidant potential in the DPPH• (IC = 0.

Bio-preservation Effect of Probiotic Lactiplantibacillus plantarum S61 Against Rhodotorula glutinis and Listeria monocytogenes in Poultry Meat.

The objective of this work is the study of the antifungal and antibacterial activity of Lactiplantibacillus plantarum S61 strains, isolated from traditional fermenting green olives against Rhodotorula glutinis UMP 22 and Listeria monocytogenes ATCC 19117, and its application in meat as bio-preservative agent. The cell-free supernatant (CFS) of Lpb. plantarum S61 shows high inhibition zones, which are 22.

Does probiotic Kefir reduce dyslipidemia, hematological disorders and oxidative stress induced by zearalenone toxicity in wistar rats?

Zearalenone (ZEA) is a toxic metabolite of the genus , which causes hepatotoxicity and induces oxidative stress. Kefir is an important probiotic dairy-product showing important antioxidant potential. In this study, the effect of Kefir supplementation to mitigate ZEA toxicity in rats was investigated.

Extraction and characterization of chitin, chitosan, and protein hydrolysate from the invasive Pacific blue crab, Portunus segnis (Forskål, 1775) having potential biological activities.

The diversity of marine biomasses is a set of exploitable and renewable resources with application in several sectors. In this context, a co-culture based on three protease-producing bacterial isolates, namely Aeribacillus pallidus VP3, Lysinibacillus fusiformis C250R, and Anoxybacillus kamchatkensis M1V strains, was carried out in a medium based on the blue swimming crab Portunus segnis bio-waste. Proteases production was optimized using a central composite design (CCD).

Multiple linear regression models to simulate spore yields of Bacillus amyloliquefaciens BS13 through optimization of medium composition.

Bacillus amyloliquefaciens is a food spoilage spore-forming bacterium. Its spores are useful for multiple biotechnological applications. Nevertheless, few reports are available regarding the achievement of a high cell density and good sporulation effectiveness under fermentation conditions.

Heterologous expression and purification of keratinase from Actinomadura viridilutea DZ50: feather biodegradation and animal hide dehairing bioprocesses.

The keratin-degrading bacterium Actinomadura viridilutea DZ50 secretes a keratinase (KERDZ) with potential industrial interest. Here, the kerDZ gene was extracellularly expressed in Escherichia coli BL21(DE3)pLysS using pTrc99A vector. The recombinant enzyme (rKERDZ) was purified and biochemically characterized.

Biochemical and molecular characterization of an acido-thermostable endo-chitinase from Bacillus altitudinis KA15 for industrial degradation of chitinous waste.

This paper reports the isolation and identification of an acido-thermostable chitinase (ChiA-Ba43) which was purified, from the culture liquid of Bacillus altitudinis strain KA15, and characterized. Purification of ChiA-Ba43 produced a 69.6-fold increase in the specific activity (120,000 U/mg) of the chitinase, with a yield of 51% using colloidal chitin as substrate.

Identification and homology modeling of a new biotechnologically compatible serine alkaline protease from moderately halotolerant Gracilibacillus boraciitolerans strain LO15.

A new serine alkaline protease (designated as SAPGB) from Gracilibacillus boraciitolerans strain LO15, was produced (9000 U/mL), purified, and characterized. SAPGB has a monomer structure with a precise molecular weight of 30,285.03 kDa as learnt from matrix-assisted laser desorption/ionization-time of flight/mass spectroscopy (MALDI-TOF/MS) exploration.

α-Amylase production by Tepidimonas fonticaldi strain HB23: statistical optimization and compatibility study for use in detergent formulations.

In a previous study, a thermostable α-amylase-producing bacterium (designated HB23) was isolated from an Algerian hydrothermal spring. In the present study, the native strain was subjected to a statistical optimization aimed at enhancing the α-amylase production. To achieve this, thirteen factors have been studied, among which are cultural and nutritional parameters.

A thermophilic and thermostable xylanase from Caldicoprobacter algeriensis: Recombinant expression, characterization and application in paper biobleaching.

A novel xylanase gene xynBCA, encoding a polypeptide of 439 residues (XynBCA), was cloned from Caldicoprobacter algeriensis genome and recombinantly expressed in Escherichia coli BL21(DE3). The amino acid sequence analysis showed that XynBCA belongs to the glycoside hydrolase family 10. The purified recombinant enzyme has a monomeric structure of 52 kDa.

A biological clean processing approach for the valorization of speckled shrimp Metapenaeus monoceros by-product as a source of bioactive compounds.

The efficiency of the proteolytic strain Anoxybacillus kamchatkensis M1V in the fermentation of speckled shrimp by-product was investigated for the recovery of a deproteinized bioactive hydrolysate. The biological activities of the resulting hydrolysate were also examined by applying several antioxidant and enzyme inhibitory assays. The strain M1V was found to produce high level of protease activity (2000 U/mL) when grown in media containing only shrimp powder at 25 g/L.

Statistical Experimental Design Optimization of Microbial Proteases Production under Co-Culture Conditions for Chitin Recovery from Speckled Shrimp By-Product.

This study was designed with the aim to produce microbial proteases in presence of speckled shrimp by-product. For this reason, three strains belonging to genus, namely, VP3, C250R, and M1V were studied under co-culture procedure. A Taguchi L27 experimental design was applied to optimize the co-culture parameters.