Impact of nonsynonymous single nucleotide polymorphisms in gene on protein stability and thrombotic risk: a molecular dynamic approach.

Introduction: Thrombosis is a serious vascular disorder influenced by genetic factors, including nonsynonymous single nucleotide polymorphisms (nsSNPs) in the gene, which encodes the endothelial protein C receptor (EPCR). These mutations may disrupt EPCR stability and impair its anticoagulant function, thereby increasing the risk of thrombosis.

Methods: We employed a multi-layered computational approach to analyze 217 nsSNPs in the gene.

Intelligent Care: A Scientometric Analysis of Artificial Intelligence in Precision Medicine.

The integration of advanced computational methods into precision medicine represents a transformative advancement in healthcare, enabling highly personalized treatment strategies based on individual genetic, environmental, and lifestyle factors. These methodologies have significantly enhanced disease diagnostics, genomic analysis, and drug discovery. However, rapid expansion in this field has resulted in fragmented understandings of its evolution and persistent knowledge gaps.

Clinico-Epidemiological Laboratory Findings of COVID- 19 Positive Patients in a Hospital in Saudi Arabia.

Background: Understanding COVID-19's onset and clinical effects requires knowing host immune responses.

Objective: To investigate the presence of IgM, IgG, and cytokine levels (IL-2 and IL-6) in individuals with COVID-19 who have had their diagnosis confirmed by PCR.

Methods: This cross-sectional research included 70 adult ICU patients from King Abdullah Hospital in Bisha, Saudi Arabia.

Emergence of , , and genes in multidrug-resistant and in Saudi Arabia.

Multidrug-resistant (MDR) patterns due to extended-spectrum β-lactamase (ESBL) production in pathogenic bacteria are now becoming prevalent in hospitals worldwide, posing a public health challenge. The aim of the present study was to determine the antibiotic susceptibility patterns and distribution of the , , and ESBL resistance genes in MDR and . A cross-sectional study was conducted between September 2017 and August 2018 in the King Abdullah Hospital (Bisha, Saudi Arabia).

Phenotypic Characterization and Antibiotic Resistance Patterns of Extended-Spectrum -Lactamase- and AmpC -Lactamase-Producing Gram-Negative Bacteria in a Referral Hospital, Saudi Arabia.

Background: Emergence of pathogenic bacteria carrying -lactamase-resistant determinants has become a major health problem in the hospital setting. The study aimed to determine antibiotic-resistant patterns and frequency of extended-spectrum -lactamase- (ESBL-) producing Gram-negative bacteria (GNB) and AmpC -lactamase-producing GNB.

Methodology: A prospective cross-sectional study was conducted during a period from September 2017 to August 2018 at King Abdullah Hospital, Bisha Province, Saudi Arabia.

MicroRNA-Based Prophylaxis in a Mouse Model of Cirrhosis and Liver Cancer.

Most hepatocellular carcinomas (HCCs) arise in the context of chronic liver disease and/or cirrhosis. Thus, chemoprevention in individuals at risk represents an important but yet unproven approach. In this study, we investigated the ability of microRNA (miRNA)-based molecules to prevent liver cancer development in a cirrhotic model.

Enteroparasitosis infections among renal transplant recipients in Khartoum state, Sudan 2012-2013.

Objectives: Renal transplantation procedure markedly increased over the past few decades. The risk of harboring parasitic diseases may affect transplant recipients during life expectancy. We aimed in this study to determine the enteroparasitosis frequency among renal transplant recipients in Khartoum state, Sudan.

miR-199a-3p Modulates MTOR and PAK4 Pathways and Inhibits Tumor Growth in a Hepatocellular Carcinoma Transgenic Mouse Model.

Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death worldwide. Prognosis is poor, and therapeutic options are limited. MicroRNAs (miRNAs) have emerged as potential therapeutic molecules against cancer.

Detection and sequencing of rotavirus among sudanese children.

Introduction: Diarrheal diseases are a big public health problem worldwide, particularly among developing countries. The current study was conducted to detect and characterize group A rotavirus among admitted children with gastroenteritis to the pediatric hospitals, Sudan.

Methods: A total of 755 stool samples were collected from Sudanese children with less than 5 years of age presenting with acute gastroenteritis during the period from April to September 2010.

Phenotypic and Genotypic Analysis of Multidrug-Resistant Isolates from Sudanese Patients.

Currently, mutations in , , and genes and promoter were considered to be involved in conferring resistance to rifampicin, isoniazid, and streptomycin in (MTB). The aims of this study were to detect the prevalence of first-line tuberculosis (TB) drug resistance among a group of previously treated and newly detected TB patients, to determine the association between prevalence of multidrug resistance (MDR) and demographic information (age and sex), to explain genes correlated with MDR , and to characterize MTB via 16S ribosomal RNA (16S rRNA) analysis. A hundred MTB isolates from Sudanese pulmonary TB patients were included in the study.

Anti-tumor activity of a miR-199-dependent oncolytic adenovirus.

The down-regulation of miR-199 occurs in nearly all primary hepatocellular carcinomas (HCCs) and HCC cell lines in comparison with normal liver. We exploited this miR-199 differential expression to develop a conditionally replication-competent oncolytic adenovirus, Ad-199T, and achieve tumor-specific viral expression and replication. To this aim, we introduced four copies of miR-199 target sites within the 3' UTR of E1A gene, essential for viral replication.

Role of microRNAs in hepatocellular carcinoma: a clinical perspective.

Hepatocellular carcinoma (HCC) is one of the most deadly tumors, and current treatments for the disease are often ineffective. The discovery of the involvement of microRNAs (miRNAs) in hepatocarcinogenesis represents an important area of investigation for the development of their clinical applications. These molecules may act as oncogenes or tumor suppressors by directly or indirectly controlling the expression of key proteins involved in cancer-associated pathways.

Liver tumorigenicity promoted by microRNA-221 in a mouse transgenic model.

Unlabelled: MicroRNA-221 (miR-221) is one of the most frequently and consistently up-regulated microRNAs (miRNAs) in human cancer. It has been hypothesized that miR-221 may act as a tumor promoter. To demonstrate this, we developed a transgenic (TG) mouse model that exhibits an inappropriate overexpression of miR-221 in the liver.

MicroRNA response to environmental mutagens in liver.

During the recent few years, microRNAs emerged as key molecules in the regulation of mammalian cell functions. It was also shown that their altered expression can promote pathologic conditions, such as cancer and other common diseases. Because environmental exposure to biological, chemical or physical agents may be responsible for human diseases, including cancer, uncovering relationships between exposure to environmental carcinogens and expression of microRNAs may help to disclose early mechanisms of disease and it may potentially lead to the development of useful indicators of toxic exposure or novel biomarkers for carcinogenicity testing.