Development of a microneedle patch for delivery of mRNA-lipid nanoparticles.

mRNA delivered by microneedle patch (MNP) can enable painless delivery, reduced need for healthcare expertise, and improved thermostability. In this study, we investigated formulation and manufacturing approaches for developing MNPs that deliver mRNA-loaded lipid nanoparticles (LNPs) encoding luciferase as a reporter protein during MNP fabrication and storage, including mRNA-LNP concentration, formulation, pH, excipients, and backing material. MNPs were assessed for mRNA-LNP size, encapsulation efficiency, and protein expression in vitro and in vivo.

Multivariate protein landscape of host response in hospitalised patients with suspected infection in the emergency department.

Suspected infection requiring hospitalisation has highly heterogenous presentation. Yet, variances in host response and its implications are largely unknown. In this multicentre cohort of 3802 individual patients presenting to the Emergency Department (ED) with suspected infection requiring hospitalisation, we apply uniform manifold approximation and projections and K-means clustering to 29 plasma proteins to identify biologically discrete host response clusters.

Non-invasive imaging-guided preclinical assessments reveal high performance photothermal effect of an Au-solid lipid nanomaterial.

With the rising global cancer crisis, photothermal therapy (PTT) has emerged as a promising treatment for solid tumours. PTT utilizes near-infrared (NIR) photons to generate localized heat through plasmonic nanoparticles. We optimized the production of a novel PTT nanomaterial, a gold-coated solid lipid nanoparticle (Au-SLN), and characterized its functionalities using non-invasive multimodality imaging.

The effect of mRNA-lipid nanoparticle composition on stability during microneedle patch manufacturing.

Messenger RNA (mRNA) encapsulated in lipid nanoparticles (LNPs) is a potent technology with broad applications. Microneedle patches (MNPs) can enhance the accessibility of mRNA-LNPs for vaccination or therapeutic applications. We evaluated the effects of LNP composition on the stability of mRNA-LNPs before and after MNP manufacturing, as assessed by changes in mRNA-LNP size, encapsulation efficiency, and protein expression in vitro and in vivo.