Genetic testing for BRCA1/2 variants in Northern African women with ovarian and breast cancers: a multicentre study of an under-represented ancestry.

Background: There is a lack of studies investigating the burden of BRCA1/2 pathogenic variants (PVs) in Northern African countries using next-generation sequencing (NGS)-based testing in patients with epithelial ovarian (EOC) and triple-negative breast cancer (TNBC).

Patients And Methods: We established a multicentre registry for genetic testing of unselected patients referred from five centres from 2019 to 2022 across Egypt. Germline or somatic BRCA1/2 sequencing was carried out by target enrichment using the AmoyDx® BRCA1/2 Mutation Kit, and sequencing was carried out using the Illumina NextSeq500 system.

T cell epitope content comparison using EpiCC correlates with vaccine efficacy against heterologous porcine reproductive and respiratory syndrome virus type 2 strains.

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most ubiquitous RNA viruses affecting pigs and pig farms globally. While vaccines are available, they are not entirely effective, and the introduction of modified live virus vaccines (MLV) has contributed to an increase in the rate of viral evolution. While vaccines induce humoral responses that may contribute to immunity against PRRSV, vaccines containing T cell epitopes that are well-matched to circulating strains are believed to be more likely to induce protective effects upon challenge or field exposure.

Inactivity-induced NR4A3 downregulation in human skeletal muscle affects glucose metabolism and translation: Insights from in vitro analysis.

Objective: Physical activity promotes health, whereas inactivity is associated with metabolic impairment. The transcription factor nuclear receptor subfamily 4 group A member 3 (NR4A3) is a pleiotropic regulator of skeletal muscle exercise adaptation and metabolism. However, the consequence of lower NR4A3 expression remains largely unexplored.

Live-cell GLUT4 translocation assay reveals Per3 as a novel regulator of circadian insulin sensitivity in skeletal muscle cells.

Type 2 diabetes (T2D) is a growing global health concern, with skeletal muscle playing a central role due to its contribution to postprandial glucose disposal. Insulin resistance in skeletal muscle often precedes the clinical onset of T2D and is characterised by impaired GLUT4 trafficking. Circadian disruption is increasingly recognised as a contributor to metabolic dysfunction, yet its impact on skeletal muscle insulin sensitivity remains poorly defined.

Variations and Challenges in the Management of Traumatic Bladder Injuries: An Experience From a Large Trauma Center.

Introduction:  Bladder trauma, often resulting from blunt or penetrating injuries, presents a significant challenge in trauma care due to its potential for serious complications and mortality. This retrospective study aimed to investigate demographic characteristics, mechanisms of injury, management strategies, and outcomes in bladder trauma patients, with a focus on comparing the differences between blunt and penetrating trauma cases.

Methods:  Data were collected from trauma patients presenting to Arrowhead Regional Medical Center between February 17, 2013, and October 30, 2022.