Plant-related quality attributes affecting FcγRIIIa binding: affinity chromatography analysis of rituximab glycovariants from .

Monoclonal antibodies (mAb) produced in plants, known as plantibodies, represent a cost-effective alternative to conventional mammalian cell cultures. Glycoengineering processes are needed to alter N-glycosylation, avoiding plant-typical glycans and enabling, for anti-cancer mAbs, the production of biobetters with improved antibody-dependent cell-mediated cytotoxicity (ADCC). In this study, glycovariants of the mouse/human chimeric anti-CD20 antibody rituximab were produced in plants by transient expression using vacuum-agroinfiltration technology and LED lighting.