A bio-guided investigation of Mitragyna inermis (Willd) O. Kuntze (Rubiaceae) unveils natural product isolates with potent cross-activity against sensitive and multidrug resistant Plasmodium falciparum strains in vitro.

Ethnobotanical Relevance: Malaria remains a significant global health challenge, especially in sub-Saharan Africa, with rising resistance to artemisinin-based therapies highlighting the urgent need for new, effective, and safer treatments from medicinal plants which have been used by the indigenous people.

Aim Of The Study: This study investigates the antiplasmodial potential of natural products from Mitragyna inermis.

Methods: Crude extracts from the stem bark and twigs of Mitragyna inermis were prepared using various solvents (water, ethanol/water, ethanol, and methanol) and tested for activity against Plasmodium falciparum strains Dd2 and 3D7 using the SYBR Green I-based fluorescence assay.