JMJD6 and YBX1 physically interact and regulate HOTAIR proximal promoter.

Earlier, we showed that jumonji domain containing protein 6 (JMJD6) interacted with HOTAIR promoter (-123 to -103 bp, termed JMJD6 interaction region [JIR]) and for maximal induction, an additional (-216 to -123 bp) region was required. In silico prediction and ENCODE data from MCF7 cells showed Y-box interacting protein 1 (YBX1) peaks in this region (YIR). Publicly available mass spectrometry data of proteins following JMJD6 immunoprecipitation identified YBX1 as an interacting partner.

Potential applications of gene expression profiles obtained from circulating extracellular vesicles in breast cancer.

Background: Liquid biopsy-based biomarkers offer several advantages since they are minimally invasive, can be useful in longitudinal monitoring of the disease and have higher patient compliance. We describe a protocol using minimal volumes of archival and prospective serum/plasma samples to define the RNA contents of EVs and discuss its benefits and limitations.

Methods: RNA-seq analysis of matched tumor biopsy, circulating EVs from breast cancer patients (EV-C, n = 26) and healthy donors (EV-H, n = 4) was performed and differentially expressed genes were validated by RT-PCR in a separate series of samples (EV-C, n = 32 and EV-H, n = 22).

JMJD6 orchestrates a transcriptional program in favor of endocrine resistance in ER+ breast cancer cells.

High expression of Jumonji domain containing protein 6 (JMJD6) is strongly associated with poor prognosis in estrogen receptor positive (ER+) breast cancer. We overexpressed JMJD6 in MCF7 cells (JOE cells) and performed RNA-seq analysis. 76% of differentially expressed genes (DEGs) overlapped with ER target genes.