A scalable and robust cationic lipid/polymer hybrid nanoparticle platform for mRNA delivery.

mRNA based gene therapies hold the potential to treat multiple diseases with significant advantages over DNA based therapies, including rapid protein expression and minimized risk of mutagenesis. However, successful delivery of mRNA remains challenging, and clinical translation of mRNA therapeutics has been limited. This study investigated the use of a lipid/polymer hybrid (LPH) nanocarrier for mRNA, designed to address key delivery challenges and shuttle mRNA to targeted tissues.

pH-Dependent Phase Behavior and Stability of Cationic Lipid-mRNA Nanoparticles.

Lipid nanoparticles (LNPs) containing mRNA can deliver genetic material to cells for use as vaccines or protein replacement therapies. We characterized the effect of solution pH on cationic LNPs containing green fluorescent protein (EGFP) mRNA and their transfection efficiency. We compared the structural and colloidal properties of mRNA LNPs with LNPs not containing mRNA and mRNA free in solution.

Synthesis, characterization and in vivo efficacy of PEGylated insulin for oral delivery with complexation hydrogels.

Purpose: This work evaluated the feasibility of combining insulin PEGylation with pH responsive hydrogels for oral insulin delivery.

Methods: A mono-substituted PEG-insulin conjugate was synthesized and purified. The site of conjugation was determined by MALDI-TOF MS.