Highly differentiated T cells link systemic and vascular inflammation in a mouse model of recurrent psoriasis.

Psoriasis is a chronic inflammatory skin-disease associated with cardiovascular comorbidities. In patients, T cells with a skin-primed phenotype are expanded in peripheral blood, indicating a role for skin to blood T cell recirculation in the development of systemic comorbidities. Here, we aimed to investigate (i) the establishment of CD4 and CD8 T cell memory, (ii) the accumulation of activated and terminally differentiated T cells, and (iii) the potential link with vascular inflammation, in a mouse model of recurrent psoriasis.

A multi-technique approach to enlighten the role of metal coordination in calcitermin antiviral properties.

In this work we presented how the use of suitable electroanalytical, thermodynamic and spectroscopic methods combined with proper experimental conditions can provide comprehensive information on the interaction between metal ions and peptides in solution, as a successful strategy for studying biological systems. Our candidate peptide is calcitermin, an effective metal chelator with significant anti-Candida and antibacterial activity in the presence of divalent metals. While the bioinorganic chemistry of calcitermin with zinc and copper is quite well described in the literature, no data about nickel complexes are available; we therefore deepened calcitermin ability to form nickel complexes by different analytical techniques, including potentiometry, ultraviolet-visible absorption spectrophotometry, circular dichroism and high-resolution mass spectrometry.

Generation of a human induced pluripotent stem cell line (UNIFEi001-A) from a patient with Spinocerebellar ataxia type 1 (SCA1).

Spinocerebellar ataxia type 1 (SCA1) is one of six SCAs related to the expansion of a CAG repeat, encoding glutamine. Induced pluripotent stem cells were generated by reprogramming fibroblasts isolated from a patient affected by SCA1, through the transduction of three viral vectors derived from a non-integrative virus. UNIFEi001-A cell line presents an ESC-like morphology, is vector-free, expresses pluripotency markers, presents a normal karyotype and can differentiate into the three germ layers.

Herpes Simplex Virus ICP27 Protein Inhibits AIM 2-Dependent Inflammasome Influencing Pro-Inflammatory Cytokines Release in Human Pigment Epithelial Cells (hTert-RPE 1).

Although Herpes simplex virus type 1 (HSV-1) has been deeply studied, significant gaps remain in the fundamental understanding of HSV-host interactions: our work focused on studying the Infected Cell Protein 27 (ICP27) as an inhibitor of the Absent-in-melanoma-2 (AIM 2) inflammasome pathway, leading to reduced pro-inflammatory cytokines that influence the activation of a protective innate immune response to infection. To assess the inhibition of the inflammasome by the ICP27, hTert-immortalized Retinal Pigment Epithelial cells (hTert-RPE 1) infected with HSV-1 wild type were compared to HSV-1 lacking functional ICP27 (HSV-1∆ICP27) infected cells. The activation of the inflammasome by HSV-1∆ICP27 was demonstrated by quantifying the gene and protein expression of the inflammasome constituents using real-time PCR and Western blot.

Effects of Flavonoids and Phenols from Leaf Extracts on Biofilm Processes in pv. .

pv. is the causal agent of black rot in crucifers, a plant disease with significant economic impact. Xanthomonadaceae is a large family of Gram-negative bacteria that cause symptoms by blocking water flow in plants by invading the xylem.

Suppressing gain-of-function proteins via CRISPR/Cas9 system in SCA1 cells.

SCAs are autosomal dominant neurodegenerative disorders caused by a gain-of-function protein with toxic activities, containing an expanded polyQ tract in the coding region. There are no treatments available to delay the onset, stop or slow down the progression of these pathologies. In this work we focus our attention on SCA1 which is one of the most common genotypes circulating in Italy.

Control of Growth by Leaf Extracts: In Vitro and in Planta Effects.

(EA) is a phytopathogenic bacterium, the causative agent of bacterial fire blight, a disease that affects Rosaceaes. In order to replace antibiotics and copper, the antimicrobial activity of three extracts of Lam., methanolic (MeOH-MOE), hydroalcoholic (HA-MOE) and hydroalcoholic with maltodextrins (HAMD-MOE), was tested on eleven strains of EA isolated from apple trees by the Emilia-Romagna Phytosanitary Department.

Effects of Leaf Extracts on pv. .

pv. (Xcc) is a Gram-negative bacterium belonging to the Xanthomonodaceae family, causing black rot in crucifers. To control this pathogen, the study investigated the effect of different leaves extracts of Lam.