Characterization of methicillin-resistant Staphylococcus aureus in Canadian hospitals: 17 years of the CANWARD study (2007-23).

Objectives: This study reviewed the patient demographic parameters, molecular characteristics and in vitro antimicrobial susceptibility testing results for MRSA isolates infecting inpatients and outpatients presenting for care to tertiary-care Canadian hospitals between 2007 and 2023.

Methods: DNA sequencing was used to generate spa types. Panton-Valentine leukocidin (PVL) genes were detected by PCR.

Novel 'Bacteriospray' Method Facilitates the Functional Screening of Metagenomic Libraries for Antimicrobial Activity.

Metagenomic techniques, notably the cloning of environmental DNA (eDNA) into surrogate hosts, have given access to the genome of uncultured bacteria. However, the determination of gene functions based on DNA sequences alone remains a significant challenge. The functional screening of metagenomic libraries represents an interesting approach in the discovery of microbial metabolites.