Publications by authors named "Anestis Tsitsos"

Beef production in Greece is a sector that has been characterized by a decline in both the output and the number of beef-producing animals over the last decades. The major challenge is low beef self-sufficiency; only 19.1% of demand is met by domestic production.

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Extensive cattle farming significantly contributes to Greece's agricultural economy. In such systems, animals mainly graze on natural grasslands whose biodiversity significantly affects meat quality traits. In Greece, the sector faces several economic challenges, while the literature investigating beef quality produced by these systems is scarce.

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The objective of this study was to assess the impact of breed and slaughter hygiene practices on beef quality traits in Northern Greece. A random sample of 159 beef carcasses from three breeds, Aberdeen Angus (AA, n = 38), Holstein (HO, n = 42), and Limousin (LI, n = 40), and crossbred (CR, n = 39) males were used. The chroma, pH, texture, chemical composition, and fatty acid profile were assessed using the muscle.

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Listeria monocytogenes, the leading cause of fatalities among foodborne pathogens, exhibits remarkable resilience in food industry environments. This study aims to isolate and characterize L. monocytogenes strains along the beef production chain of a vertically integrated industry.

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Extended-spectrum-β-lactamase (ESBL) and carbapenemase-producing Enterobacterales and Acinetobacter spp. are important nosocomial pathogens that are frequently isolated from patients and food matrices. Nevertheless, comprehensive data on the prevalence, spatiotemporal variations, and characterization of β-lactam-resistant bacteria in poultry meat products are limited.

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Extended-spectrum-β-lactamase (ESBL) and carbapenemase-producing , and spp. are associated with hospital-acquired infections and are commonly isolated across the poultry food production chain. Comprehensive data regarding the prevalence, spatiotemporal variations, and characterization of β-lactam-resistant bacteria in poultry farms and slaughterhouses is scarce.

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Article Synopsis
  • Beef is primarily produced from beef cattle, but dairy calves can also be raised for meat; this study focuses on how oregano oil in diets affects meat quality in Holstein bulls.
  • A total of 50 Holstein bulls were split into two groups: one received a standard diet, while the other’s diet was enriched with oregano oil for 90 days.
  • The results showed that the oregano oil group had better meat color and flavor scores, lower malondialdehyde levels, and higher nutritional value, indicating that oregano oil can enhance the quality of meat from Holstein bulls.
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  • Extended-spectrum-β-lactamase (ESBL) and carbapenemase-producing Enterobacterales in hospital kitchens are associated with antibiotic resistance, presenting risks for patient infections.
  • This study aimed to evaluate the prevalence and characteristics of β-lactam-resistant strains from kitchen environments and hospital staff in two Greek hospitals.
  • Findings show a notable prevalence of resistant strains in kitchens and a connection between kitchen and patient strains, indicating that kitchen environments may act as reservoirs for these harmful pathogens.
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  • This research explores 3D-printed antifungal buccal films (BFs) made from a zein-PVP polymer blend as a possible substitute for traditional antifungal oral gels, highlighting their ease of manufacturing and suitability for children.
  • The films provide sustained release, with 80% of the drug miconazole being released over 2 hours, and disintegrate in under 10 minutes while adhering to buccal tissue.
  • Comprehensive evaluations confirm the films' physicochemical stability, mucoadhesion, and effective antifungal activity, making them a promising option for treating fungal infections in the oral cavity.
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The aim of the study was to investigate in vitro the antibacterial activity of 8 commercial drinking water additives against major zoonotic poultry pathogens ( spp., , Typhimurium, and spp.).

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Article Synopsis
  • Antimicrobial resistance (AMR), particularly regarding gram-negative bacteria and extended-spectrum β-lactamases (ESBL), poses significant health risks in humans and veterinary medicine, and is a key concern in the "One Health" framework.
  • A study conducted at Aristotle University of Thessaloniki collected 500 raw poultry skin samples, finding that 64% were positive for ESBL-producing bacteria, highlighting a substantial presence of antibiotic-resistant pathogens in the food supply.
  • Among the isolates, 71.67% were identified as true ESBL producers, with specific β-lactamase genes detected, indicating a potential risk for the transmission of antibiotic resistance from poultry to humans.
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Identifying and monitoring the efficiency of alternative biocides that are presently used in livestock is gaining vast attention. The objective of this study was to determine, in vitro, the antibacterial activity of nine commercial water disinfectants, acidifiers, and glyceride blends against clinical isolates or reference strains of zoonotic pathogens belonging to the genera spp., spp.

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Edible chitosan or alginate coatings and their combinations with oregano essential oil or olive oil, have been examined for their effect on the microbiological, physicochemical and organoleptic characteristics of mutton. The results indicated that these edible coatings can contribute to maintaining good quality characteristics and extending mutton shelf-life. The total mesophilic counts in mutton ranged from 3.

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Meat quality dictates consumer preferences with hygiene forming a key component, especially in meat types with declining popularity, such as sheep and goat meat. Aiming to increase the marketability of sheep and goat meat, we examined 370 sheep and goat carcasses from two abattoirs in Greece. Tests included enumeration of the total mesophilic viable count, total psychrophilic viable count and coliform count, and detection of spp.

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Sheep farming in Greece is focused on milk production. Meat is considered a by-product and consists mainly light carcasses of undefined quality. The main challenge of the sector is to ensure sustainability, and hence efforts are towards efficient use of available resources, including undervalued carcasses of local fat-tailed sheep.

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Treatment failure of endodontic infections and their concurrent inflammations is commonly associated with microbial persistence and reinfection, also stemming from the anatomical restrictions of the root canal system. Aiming to address the shortcomings of current treatment options, a fast-disintegrating nanofibrous film was developed for the intracanal coadministration of an antimicrobial (ZnO nanoparticles) and an anti-inflammatory (ketoprofen) agent. The electrospun films were fabricated based on polymers that dissolve rapidly to constitute the actives readily available at the site of action, aiming to eliminate both microbial infection and inflammation.

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Background: Urinalysis is not routinely used in bovine medicine, and there is no evidence as to whether urine protein-to-creatinine ratio (UPC) could be used for the diagnosis of renal diseases in cattle.

Objective: The goal of the study was to determine alterations in UPCs observed with different subclinical renal diseases in clinically healthy cattle and to investigate whether UPC can efficiently differentiate cattle with and without subclinical renal pathology.

Methods: Kidney and urine samples from 57 clinically healthy adult dairy (44) and beef (13) cattle were collected after slaughter.

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