Ancient host-associated microbes obtained from mammoth remains.

Ancient genomic studies have extensively explored human-microbial interactions, yet research on non-human animals remains limited. In this study, we analyzed ancient microbial DNA from 483 mammoth remains spanning over 1 million years, including 440 newly sequenced and unpublished samples from a 1.1-million-year-old steppe mammoth.

Host Traits Impact the Outcome of Metagenomic Library Preparation From Dental Calculus Samples Across Diverse Mammals.

Dental calculus metagenomics has emerged as a valuable tool for studying the oral microbiomes of humans and a few select mammals. With increasing interest in wild animal microbiomes, it is important to understand how widely this material can be used across the mammalian tree of life, refine the related protocols and understand the expected outcomes and potential challenges of dental calculus sample processing. In this study, we significantly expand the breadth of studied host species, analysing laboratory and bioinformatics metadata of dental calculus samples from 32 ecologically and phylogenetically diverse mammals.

Stable isotope analysis of ectoparasites as a tool for understanding trophic interactions with mammalian hosts.

Climate change is expected to expand the geographic ranges of ectoparasites, increasing the transmission of vector-borne diseases and necessitating a better understanding of ectoparasite-host trophic dynamics. Haematophagous ectoparasites can serve as valuable subsamples of their hosts, retaining isotopic values that reflect dietary information in both their blood meals and tissues. However, differences in the life histories and feeding strategies of lice, fleas and ticks may influence how host isotopic composition is preserved.

Craniological differentiation amongst Southeast Asian small cats.

Nine small felid species, including the introduced domestic cat, inhabit Southeast Asia. We analysed their skull morphology, using 36 selected morphometric measurements of up to 465 specimens. Of the species examined, the fishing cat and the Asian golden cat were the largest, whilst the mainland and Sunda leopard cats were the smallest.

Multiple bursts of speciation in Madagascar's endangered lemurs.

Lemurs are often cited as an example of adaptive radiation, as more than 100 extant species have evolved and filled ecological niches on Madagascar. However, recent work suggests that lemurs lack a hallmark of other adaptive radiations: explosive speciation rates that decline over time. Thus, characterizing the tempo and mode of evolution in lemurs can reveal alternative ways that hyperdiverse clades arise over time, which might differ from traditional models.

The domestication of the wolf larynx-testing the neural crest connection.

The neural crest domestication syndrome (NCDS) hypothesis suggests that selection pressures for tameness during animal domestication resulted in a down-regulation of neural crest cell migration and proliferation, providing a mechanistic explanation for traits commonly observed across domesticates. We test the viability of this hypothesis via a derived prediction. According to the NCDS hypothesis, neural crest-derived structures should be reduced throughout the entire organism in domesticated variants compared to their wild ancestors.

The blood-sucking tick Ixodes hexagonus reveals dietary stable isotope signatures of mammalian hosts.

Ticks are obligate haematophagous ('blood-sucking') ectoparasites that are capable of retaining host dietary traces post-moult, providing an opportunity to investigate parasite-host interactions and explore their potential as non-invasive subsampling techniques. However, research on the preservation of biochemical host signatures within whole engorged parasites remains limited. Here, we examine stable isotope ratios of nitrogen (δ15N) and carbon (δ13C) across different tick tissues (exoskeleton vs.

A web-based tool for rapid and accurate craniometric differentiation of clouded leopard species.

The illegal wildlife markets of Southeast Asia are bolstered by organised criminal networks and the region's rich density of charismatic wildlife. Forensic tools identifying species and their origins are vital to combat wildlife crime. However, many require expensive technology and skilled personnel, limiting their use in rural trade hotspots.

An osteobiography of a celebrity chimpanzee reflects the changing roles of modern zoos.

The role of zoos has shifted markedly from their origins in the nineteenth century to the present day. Long-lived and charismatic individual animals have been central to zoological collections, as priorities have changed from a primary focus on entertainment to a greater emphasis on education, conservation, research and welfare. Here, we construct the osteobiography of a celebrity chimpanzee to highlight the changing practices of modern zoos over a near half-century timescale.

Genomic Insights Into Red Squirrels in Scotland Reveal Loss of Heterozygosity Associated With Extreme Founder Effects.

Remnant populations of endangered species often have complex demographic histories associated with human impact. This can present challenges for conservation as populations modified by human activity may require bespoke management. The Eurasian red squirrel, (L.

Morphological change in an isolated population of red squirrels () in Britain.

The mechanical properties of dietary items are known to influence skull morphology, either through evolution or by phenotypic plasticity. Here, we investigated the impact of supplementary feeding of peanuts on the morphology of red squirrels () from five populations in Britain (North Scotland, Borders, Jersey and two temporally distinct populations from Formby (Merseyside)). Stable isotope analysis confirmed dietary ecology in 58 specimens.

The return of raptors to Scotland's skies: Investigating the diets of reintroduced red kites and white-tailed eagles using stable isotopes.

Species reintroductions are increasingly seen as important methods of biodiversity restoration. Reintroductions of red kites Milvus milvus and white-tailed eagles Halieaeetus albicilla to Britain, which were extirpated in the late 19th and early 20th centuries, represent major conservation successes. Here, we measured stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) in feather keratin and bone collagen of museum specimens of red kites and white-tailed eagles, which were collected from across Scotland between the 1800s and 2010s.

Ecological and anthropogenic effects on the genomic diversity of lemurs in Madagascar.

Ecological variation and anthropogenic landscape modification have had key roles in the diversification and extinction of mammals in Madagascar. Lemurs represent a radiation with more than 100 species, constituting roughly one-fifth of the primate order. Almost all species of lemurs are threatened with extinction, but little is known about their genetic diversity and demographic history.

Describing the musculature of mystacial pads in harbour seals (Phoca vitulina) using diceCT.

Pinnipeds have long, sensitive, moveable mystacial vibrissae. In other mammals, intrinsic muscles contribute to protracting the vibrissae. However, the mystacial muscles of pinnipeds have not yet been systematically described.

A Computed Tomographic Study of the Molar Teeth of spp.

A photographic and computed tomography (CT) scanning study was carried out on 295 molar teeth of 18 adult male skulls and 8 skulls of including seven adult males and one adult female. The occlusal morphology of the permanent maxillary and mandibular molar teeth of was very similar to that of . Most maxillary molar teeth had six roots, with small numbers of teeth having four, five or seven roots.

The genome of the black-footed cat: Revealing a rich natural history and urgent conservation priorities for small felids.

Habitat degradation and loss of genetic diversity are common threats faced by almost all of today's wild cats. Big cats, such as tigers and lions, are of great concern and have received considerable conservation attention through policies and international actions. However, knowledge of and conservation actions for small wild cats are lagging considerably behind.

Getting to the Meat of It: The Effects of a Captive Diet upon the Skull Morphology of the Lion and Tiger.

Zoo animals are crucial for conserving and potentially re-introducing species to the wild, yet it is known that the morphology of captive animals differs from that of wild animals. It is important to know how and why zoo and wild animal morphology differs to better care for captive animals and enhance their survival in reintroductions, and to understand how plasticity may influence morphology, which is supposedly indicative of evolutionary relationships. Using museum collections, we took 56 morphological measurements of skulls and mandibles from 617 captive and wild lions and tigers, reflecting each species' recent historical range.

Complex Evolutionary History With Extensive Ancestral Gene Flow in an African Primate Radiation.

Understanding the drivers of speciation is fundamental in evolutionary biology, and recent studies highlight hybridization as an important evolutionary force. Using whole-genome sequencing data from 22 species of guenons (tribe Cercopithecini), one of the world's largest primate radiations, we show that rampant gene flow characterizes their evolutionary history and identify ancient hybridization across deeply divergent lineages that differ in ecology, morphology, and karyotypes. Some hybridization events resulted in mitochondrial introgression between distant lineages, likely facilitated by cointrogression of coadapted nuclear variants.

Genetic swamping of the critically endangered Scottish wildcat was recent and accelerated by disease.

The European wildcat population in Scotland is considered critically endangered as a result of hybridization with introduced domestic cats, though the time frame over which this gene flow has taken place is unknown. Here, using genome data from modern, museum, and ancient samples, we reconstructed the trajectory and dated the decline of the local wildcat population from viable to severely hybridized. We demonstrate that although domestic cats have been present in Britain for over 2,000 years, the onset of hybridization was only within the last 70 years.

Limited historical admixture between European wildcats and domestic cats.

Domestic cats were derived from the Near Eastern wildcat (Felis lybica), after which they dispersed with people into Europe. As they did so, it is possible that they interbred with the indigenous population of European wildcats (Felis silvestris). Gene flow between incoming domestic animals and closely related indigenous wild species has been previously demonstrated in other taxa, including pigs, sheep, goats, bees, chickens, and cattle.

How genomic insights into the evolutionary history of clouded leopards inform their conservation.

Clouded leopards ( spp.), a morphologically and ecologically distinct lineage of big cats, are severely threatened by habitat loss and fragmentation, targeted hunting, and other human activities. The long-held poor understanding of their genetics and evolution has undermined the effectiveness of conservation actions.

A global catalog of whole-genome diversity from 233 primate species.

The rich diversity of morphology and behavior displayed across primate species provides an informative context in which to study the impact of genomic diversity on fundamental biological processes. Analysis of that diversity provides insight into long-standing questions in evolutionary and conservation biology and is urgent given severe threats these species are facing. Here, we present high-coverage whole-genome data from 233 primate species representing 86% of genera and all 16 families.