Geobotanical Study, DNA Barcoding, and Simple Sequence Repeat (SSR) Marker Analysis to Determine the Population Structure and Genetic Diversity of Rare and Endangered L.

The ongoing genetic erosion of natural populations in their native habitats underscores the urgent need for targeted conservation and restoration strategies. This study provides the first comprehensive characterization of populations in the Almaty region of Kazakhstan, integrating morphological descriptors (46 parameters), molecular markers, geobotanical, and remote sensing analyses. Geobotanical and remote sensing analyses enhanced understanding of accession distribution, geological features, and ecosystem health across sites, while also revealing their vulnerability to various biotic and abiotic threats.

Epidemiological and molecular analysis of anthrax cases of the Zhambyl region Kazakhstan in 2023.

Background: Anthrax, caused by , continues to pose a serious zoonotic threat in endemic areas such as southern Kazakhstan. Its persistence in the environment through resilient spores facilitates prolonged transmission cycles between animals and humans.

Objective: This study aimed to characterize the epidemiological, clinical, and molecular features of human anthrax cases reported in the Zhambyl region of Kazakhstan during 2023.

Molecular prevalence of in sheep exhibiting neurological symptoms in Kazakhstan.

Coenurosis, caused by the larval stage of - , is a severe parasitic disease that affects the central nervous system of sheep and causes significant economic losses in livestock farming. This study aimed to determine the molecular prevalence of in sheep with neurological symptoms in Kazakhstan using conventional PCR followed by Sanger sequencing. The study was conducted in the West Kazakhstan and Abay regions.

First study on molecular identification of in sheep in southern Kazakhstan.

Background And Aim: Anaplasmosis in small ruminants is a tick-borne infection caused mainly by the obligate intraerythrocytic bacterium . It is usually subclinical, with persistent infection in affected animals, but acute disease can occur, particularly in young animals. The pathogen is widespread in Central Asia and neighboring regions.

Complete genome sequence of a human influenza a virus (H1N1) detected in Kazakhstan in the fall of 2022.

An virus strain, ), was isolated in Karaganda, Central Kazakhstan during a study of acute respiratory infections among hospital inpatients in 2022. Here, we present the complete genome sequence of this strain.

Kazakhstan tulips: comparative analysis of complete chloroplast genomes of four local and endangered species of the genus L.

Species of are important ornamental plants used for horticultural purposes in various countries, across Asia, Europe, and North Africa. The present study is the first report on typical features of the complete chloroplast genome sequence of four local and endangered species including , and from Kazakhstan using Illumina sequencing technology. The comparative analyses revealed that the complete genomes of four species were highly conserved in terms of total genome size (152.

Assessing Genetic Variability and Population Structure of (Black Alder) in Kazakhstan Using SSR Markers.

plays a crucial role in flood control, riverbank stabilization, and water purification. Recognized for its ecological significance, it is listed in the Red Book of Kazakhstan. This study investigated the genetic variability of populations in Kazakhstan, analyzing 78 trees from seven populations in the Bayanaul mountain forest massif and the northern Turgay regions using 12 SSR markers.

Molecular Characterization of spp. in Cattle from Kazakhstan.

Bovine anaplasmosis is an infectious vector-borne disease caused by bacteria of the genus , which have a wide global distribution and represent a high economic burden for agriculture. The use of molecular genetic techniques has increased our knowledge of the species diversity of spp. and naturally susceptible animals.

PCR analysis: a comprehensive bioinformatics tool for enhancing nucleic acid amplification assays.

Nucleic acid amplification assays represent a pivotal category of methodologies for targeted sequence detection within contemporary biological research, boasting diverse utility in diagnostics, identification, and DNA sequencing. The foundational principles of these assays have been extrapolated to various simple and intricate nucleic acid amplification technologies. Concurrently, a burgeoning trend toward computational or virtual methodologies is exemplified by PCR analysis.

A novel recombinant surface protein as an antigen in indirect enzyme-linked immunosorbent assay for the serological diagnosis of tropical theileriosis.

Background And Aim: infection in cattle causes major economic losses in livestock production in many Central Asian countries, including the southern region of Kazakhstan. This study aimed to obtain a recombinant surface protein (TaSP) and to investigate its possible use as an antigen in an indirect enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of bovine theileriosis.

Materials And Methods: Recombinant TaSP was obtained by cloning a polymorphic region of the TaSP gene, expressing it in strain BL21, and purifying it by metal chelating chromatography.

Molecular identification of (Gedoelst, 1920) from the Eurasian badger () and ascarids from other wild carnivores in Kazakhstan.

Introduction: The presence of gastrointestinal nematodes, including zoonotic ascarids, in wild canids, felids and mustelids as definitive hosts in Central Asian countries has been documented in many studies based on traditional morphological methods. In contrast, relevant data for the badger are scarce. The aim of this study was the molecular identification of ascarid nematodes from five wild carnivore species in different regions of Kazakhstan.

Molecular evaluation of spp. Infection in the Volga-Ural Saiga antelope population of the Republic of Kazakhstan.

Saiga antelope () is a protected species in Kazakhstan. Little is known about the parasitofauna of these mammals. Therefore, the focus of this study was to evaluate the prevalence and species diversity of spp.

Non-industrial production of therapeutic lentiviral vectors: How to provide vectors to academic CAR-T.

Bringing effective cancer therapy in the form of chimeric antigen receptor technology to untapped markets faces numerous challenges, including a global shortage of therapeutic lentiviral or retroviral vectors on which all current clinical therapies using genetically modified T cells are based. Production of these lentiviral vectors in academic settings in principle opens the way to local production of therapeutic cells, which is the only economically viable approach to make this therapy available to patients in developing countries. The conditions for obtaining and concentrating lentiviral vectors have been optimized and described.

Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan.

Background And Aim: Infectious bovine keratoconjunctivitis (IBK) causes a significant economic loss to cattle industries in many countries, including Kazakhstan. Although is recognized as an etiologic agent of IBK, other bacterial and viral agents have been suspected to play a role in the pathogenesis of this disease. This study aimed to evaluate samples collected from the eyes of IBK-affected cattle in Eastern Kazakhstan at different stages of IBK for the presence of , , , , and Bovine Herpes Virus Type 1 (BHV-1) and to characterize gene sequence diversity from positive samples.

Identification of the causative agent of canine babesiosis in the North of Kazakhstan.

Background: Canine babesiosis is a common disease in the northern part of the Republic of Kazakhstan, in particular in the Kostanay region. In recent years, a large number of cases of the disease with a variety of clinical symptoms have been registered.

Aim: The purpose of the study was to monitor the spread, characterization, and identify the species involved of species in ticks and blood of dogs in the Kostanai region.

Draft genome sequence of a strain isolated from a patient in Kazakhstan with colorectal cancer.

is an invasive obligate anaerobe found in the oral microbiota and associated with colorectal cancer. Here, we announce the draft genome sequence of strain Fn11kaz from a patient with colorectal cancer in Kazakhstan.

A survey of the parasites of Ural saiga antelopes and Turkmenian kulans of Kazakhstan.

Saiga antelope and Turkmenian kulans are considered critically endangered and near threatened, respectively, by the International Union for Conservation of Nature (IUCN). Due to these species' fragile status, it is important to understand the pathogens infecting their remaining populations. A total of 496 faecal samples were collected from Ural saiga antelope in western Kazakhstan during June, September, and November of 2021 and May and August of 2022 and 149 faecal samples were collected from kulans in the Altyn-Emel nature reserve in south-eastern Kazakhstan from June to August of 2021.

in Kazakhstan, population structure and comparison with worldwide genetic diversity.

is the main causative agent of brucellosis in cattle, leading to severe economic consequences in agriculture and affecting public health. The zoonotic nature of the infection increases the need to control the spread and dynamics of outbreaks in animals with the incorporation of high resolution genotyping techniques. Based on such methods, is currently divided into three clades, A, B, and C.

Theileria and Babesia infection in cattle - First molecular survey in Kazakhstan.

Central Asia, including Kazakhstan, is an endemic area of Theileria and Babesia infections in cattle. Current data on the geographic distribution, prevalence, and genetic diversity of these pathogens in vertebrate hosts are lacking in Kazakhstan. The present study aimed to fill this gap, using molecular techniques for the first time.

Molecular Characterisation and Phylogeny of Tula Virus in Kazakhstan.

Orthohantaviruses are zoonotic pathogens that play a significant role in public health. These viruses can cause haemorrhagic fever with renal syndrome in Eurasia. In the Republic of Kazakhstan, the first human cases were registered in the year 2000 in the West Kazakhstan region.

Phylogeography: New Clues From Kazakhstan, Central Asia.

This article describes strains isolated in Kazakhstan since the 1950s until year 2016 from sixty-one independent events associated with anthrax in humans and animals. One hundred and fifty-four strains were first genotyped by Multiple Locus VNTR (variable number of tandem repeats) Analysis (MLVA) using 31 VNTR loci. Thirty-five MLVA31 genotypes were resolved, 28 belong to the A1/TEA group, five to A3/Sterne-Ames group, one to A4/Vollum and one to the B clade.