Integrative taxonomy suggests hidden diversity within the fish genus Cyttopsis (Zeiformes, Parazenidae).

Nine specimens of Cyttopsis rosea (Zeiformes: Parazenidae) were collected during scientific surveys at three different locations in the northeast Atlantic. All nine specimens were included in the molecular analysis, adding new cytochrome c oxidase subunit I sequences to public databases. Of these, six specimens were retained for detailed morphological examination.

Diving deeper into the taxonomy of the Neoscopelus species complex (Myctophiformes: Neoscopelidae) with the description of Neoscopelus serranoi sp. nov.

Previous studies have highlighted possible cryptic biodiversity in the genus Neoscopelus. This hypothesis was tested using new morphological, molecular and biogeographical data on species of this genus caught in the north Atlantic between 2010 and 2022. The information obtained has been combined with available data in an integrative approach, including a review of morphological characters reported in the ichthyological literature and DNA-based species delimitation analyses.

Second world record for Barathronus roulei Nielsen, 2019 (Ophidiiformes, Bythitidae), from the Porcupine Bank (Northeast Atlantic).

Barathronus is a genus of blind cusk eels comprising 11 valid species. In this paper, we report the second specimen ever documented of Barathronus roulei (Bythitidae) obtained from the Porcupine Bank by R.V.

Unveiling taxonomic diversity in the deep-sea fish genus Notacanthus (Notacanthiformes: Notacanthidae) with description of Notacanthus arrontei n. sp.

Notacanthid fishes constitute a common part of benthopelagic deep-sea fish communities on seamounts and continental slopes around the world. However, their highly conserved morphology and the usual lack of information on deep-water organisms make it difficult to appropriately address their biodiversity. A multidisciplinary approach combining morphological data with a DNA-based species delimitation analyses was used to explore the taxonomy of Notacanthus species.

On the occurrence of a post-larval specimen of Brosme brosme (Gadiformes: Lotidae) on Porcupine Bank (west Ireland).

The occurrence of a small specimen of Brosme brosme (Gadiformes: Lotidae) from the Porcupine Bank is reported. A single specimen with a total length of 73.2 mm was caught with bottom trawl at a depth of 322 m depth in 2017.

DNA Barcoding of the Genus (Aulopiformes: Paralepididae) with a Coastal Record and Biological Features of .

One specimen of the duckbill barracudina of 402 mm TL was caught in a shallow coastal area in Galician waters, northwest of Spain. Morphometric and meristic parameters along with DNA barcoding, based on cytochrome c oxidase subunit I, were used to confirm the specimen identity. Neighbor-joining analysis of nominal sequences of the genus obtained from the Barcode of Life Data System indicates the presence of six representative groupings of potential species, in contrast to the three that are currently recognized as valid.

(Gadiformes: Gaidropsaridae), a New Northeast Atlantic Rockling Fish, with Commentary on the Taxonomy of the Genus.

A new species of rockling fish genus is described based on six specimens collected in Galicia and Porcupine Banks, in Atlantic European waters. An analysis of morphological characters has confirmed the specific status of specimens of a previously described clade by comparison of DNA sequences. sp.

Identification of the shell-boring parasite Polydora hoplura (Annelida: Spionidae) on wild stocks of Pecten maximus in Galician waters, NW Spain.

Infestations by shell-boring polychaetes have been gaining attention in recent years due to the harmful effects that their presence can pose to the growth and production of marine bivalves worldwide. The Galician region, located in the north-western corner of the Iberian Peninsula, is one of the major producers of marine bivalves in the word and therefore highly dependent on this industry. The recent finding of an unknown polychaete boring into the shells of the king scallop, Pecten maximus, has raised the attention of producers and authorities due to its potential harm to exploitable populations and the economical losses that could entail.

Unexpected deep-sea fish species on the Porcupine Bank (NE Atlantic): Biogeographical implications.

Four specimens corresponding to three rare deep-water fish species were caught on the Porcupine Bank (Northeast Atlantic) in September 2019. These catches include the new northernmost records of Azores rockling Gaidropsarus granti and deep-water dab Poecilopsetta beanii in the Atlantic Ocean and the second record of the latter species in its eastern zone. Three of the specimens were retained and their molecular identification also allowed the Cataetyx alleni DNA barcode to be obtained for the first time.

Apparently contradictory routes in the expansion of two fish species in the Eastern Atlantic.

One specimen of the boreal Clupea harengus (Clupeidae) was caught southerly, in the Gulf of Cádiz (southern Spain), whereas a specimen of the subtropical Epinephelus aeneus (Serranidae) was caught northerly, in Galician waters (north-west of Spain). These catches represent a new southernmost record in the eastern Atlantic for C. harengus and the second northernmost for E.

Geographic range expansion of Ephippion guttifer (Tetraodontidae) in the north-eastern Atlantic.

The first record of the prickly puffer Ephippion guttifer (Tetraodontidae) from Galician waters (north-west Spain) is reported based on a male specimen of 570 mm total length (L ) caught in the Ría de Vigo. Morphometric, meristic and DNA barcode data confirmed the identification. Histological examination of reproductive tissue was carried out in this species for the first time, showing a mature male in an actively spawning phase.

Phylogenetic analysis shows the general diversification pattern of deep-sea notacanthiforms (Teleostei: Elopomorpha).

The Notacanthiformes is an ancient group of deep-sea ray-finned fishes comprising 27 species in two families; Halosauridae and Notacanthidae. Although many studies have tried to reconstruct the phylogenetic relationships among the major clades of Elopomorpha, little is known about the evolutionary history of notacanthiforms. Molecular and morphological data were used to test previous hypotheses regarding the phylogenetic relationships among notacanthiform taxa, and to unravel the origin and evolution of this group.

Halosaur fishes (Notacanthiformes: Halosauridae) from Atlantic Spanish waters according to integrative taxonomy.

From 2009 to 2011 thirty-five specimens belonging to six halosaurid species of the family Halosauridae were captured in two different locations in the northern waters of Spain. The specimens were identified as belonging to the genera Halosauropsis Collett, 1896, Halosaurus Johnson, 1864 and Aldrovandia Goode & Bean, 1896, including the following species: Halosauropsis macrochir (Günther, 1878), Halosaurus ovenii Johnson, 1864, Halosaurus johnsonianus Vaillant, 1888, Aldrovandia affinis (Günther, 1877), Aldrovandia phalacra (Vaillant, 1888) and Aldrovandia oleosa Sulak, 1977. The morphometric measurements and meristic characters of these specimens are given.

Taxonomic study of Bathygadidae fishes (Gadiformes) from Atlantic Spanish waters combining morphological and molecular approaches.

From 2009 to 2011 eleven specimens belonging to four bathygadid species of the family Bathygadidae were captured in two different locations in the northern waters of Spain. The morphometric measurements and meristic characters of these specimens are given. The specimens were identified as belonging to the genera Gadomus Regan, 1903, and Bathygadus Günther, 1878, including the following species: Gadomus dispar (Vaillant, 1888), Gadomus longifilis (Goode & Bean, 1885), Gadomus arcuatus (Goode & Bean, 1886) and Bathygadus melanobranchus Vaillant, 1888.

Phylogenetic comparison of opine dehydrogenase sequences from marine invertebrates.

Three cDNA sequences encoding putative opine dehydrogenase (OpDH) enzymes from the mussel Mytilus galloprovincialis were obtained. The deduced amino acid sequences were clearly distinguishable from each other, showing that several OpDH transcripts could occur in the mussel tissues (p distance 0.46-0.

Identification of octopine dehydrogenase from Mytilus galloprovincialis.

A cDNA encoding the putative octopine dehydrogenase (OcDH) from the mussel Mytilus galloprovincialis was cloned and sequenced. The complete coding region was expressed in the bacteria Escherichia coli and the recombinant protein was purified. The M.

A microsatellite genetic map of the turbot (Scophthalmus maximus).

A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites were mapped in 26 linkage groups, six markers remaining unlinked.

Combined use of established and novel tumour markers in the diagnosis of head and neck squamous cell carcinoma.

Serum SCC, CYFRA 21-1, and CEA are the common tumour markers for head and neck squamous cell carcinoma (HNSCC), although diagnostic sensitivity should be yet improved, especially at early stages. In the present study, we have reported the diagnostic value of two novel serum tumour markers in HNSCC: alpha-L-fucosidase (AFU) activity, and total sialic acid concentration adjusted by total protein concentration (TSA/TP). Using the cut-off 4.

Purification of human alpha-L-fucosidase precursor expressed in Escherichia coli as a glutathione S-transferase fusion protein.

Alpha-L-fucosidase (FUC) is a glycosidase involved in the degradation of fucose-containing glycoconjugates. A cDNA representing the complete sequence of human FUC was inserted into the prokaryotic expression vector pGEX-2T. High levels of the glutathione S-transferase (GST) fusion protein were detected in Escherichia coli cells after induction with isopropyl thio-beta-D-galactopyranoside.

Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies.

A method to improve the reactivity to specific lectins of N-glycoproteins isolated from human colorectal mucosa and from adenocarcinoma biopsies was developed using a combination of techniques. Total protein extracts were subjected to affinity chromatography, using the immobilised lectin Concanavalin A coupled to Sepharose, by fast performance liquid chromatography (FPLC). N-glycoprotein enriched fractions were resolved by SDS-PAGE, transferred to PVDF membranes and incubated with various lectins.