Publications by authors named "Alberto Robazza"

Pyrolysis of lignocellulosic biomass commonly produces syngas, a mixture of gases such as CO, CO and H, as well as an aqueous solution generally rich in organic acids such as acetate. In this study, we evaluated the impact of increasing acetate shock loads during syngas co-fermentation with anaerobic microbiomes at different pH levels (6.7 and 5.

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Article Synopsis
  • The stability of plasmids in microbial cells is crucial for efficient industrial biocatalysis, as these multicopy systems offer better product outcomes compared to genomic integrations.
  • The study focuses on H16, a bacterium capable of converting inorganic carbon from CO fixation into valuable products, which has struggled with plasmid stability.
  • Researchers developed a plasmid addiction system that stabilized a multicopy plasmid, allowing H16 to successfully produce approximately 10 g/L of mevalonate with carbon yields around 25%, marking a record for C6 compounds from C1 feedstocks.
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The anaerobic digestion of aqueous condensate from fast pyrolysis is a promising technology for enhancing carbon and energy recovery from waste. Syngas, another pyrolysis product, could be integrated as a co-substrate to improve process efficiency. However, limited knowledge exists on the co-fermentation of pyrolysis syngas and aqueous condensate by anaerobic cultures and the effects of substrate toxicity.

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Hybrid thermochemical-biological processes have the potential to enhance the carbon and energy recovery from organic waste. This work aimed to assess the carbon and energy recovery potential of multifunctional processes to simultaneously sequestrate syngas and detoxify pyrolysis aqueous condensate (PAC) for short-chain carboxylates production. To evaluate relevant process parameters for mixed culture co-fermentation of syngas and PAC, two identical reactors were run under mesophilic (37 °C) and thermophilic (55 °C) conditions at increasing PAC loading rates.

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