Publications by authors named "Akiko Kanayama Katsuse"
Earlobes, nasal cavities, and fingers of 145 healthcare workers in convalescent and rehabilitation hospital (60 nurses and 85 rehabilitation healthcare workers) were sampled. Of the 3 sites sampled, Staphylococcus aureus was detected in one or more sites in 25 nurses and 27 rehabilitation workers. S.
View Article and Find Full Text PDFPopulation Analysis of Daptomycin-non-Susceptible Methicillin-Resistant Staphylococcus aureus Reveals the Presence of Variants That Contribute to Daptomycin Resistance.
Curr Microbiol
January 2024
Article Synopsis
- The study focused on analyzing the population of methicillin-resistant Staphylococcus aureus (MRSA) before and after extended treatment with daptomycin (DAP), specifically looking for subpopulations with varying resistance levels to DAP.
- A new DAP non-susceptible MRSA variant (D2) emerged from an initially DAP-susceptible strain (D1) during wound treatment, and researchers conducted bacteriological and genetic tests on these resistant variants.
- Results indicated that prolonged DAP treatment can foster the growth of resistant MRSA subpopulations, with one variant showing enhanced pigment production and a two-fold increase in resistance, highlighting the potential for slow-growing variants to develop resistance under antibiotic pressure.
Objectives: The aim of this study was to explore the origin of the PenA mosaic amino acid sequence in the ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone.
Methods: The penA sequences of 27 Neisseria subflava pharyngeal isolates were determined by the Sanger method and penA sequences of 52 isolates from nine Neisseria species were obtained from the NCBI database. Comparative analysis of each PenA sequence was performed by multiple sequence alignment using ClustalW.
Background: The ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone was first discovered in Japan in 2015.
Objectives: We investigated the possibility of horizontal gene transfer from Neisseria subflava harbouring the mosaic-like PBP-2 in the emergence of the FC428 clone. We also analysed whether there were fitness costs associated with the sustained international dissemination of the clone.
Does sub-culturing of positive MRSA blood cultures affect vancomycin MICs?
J Med Microbiol
August 2020
. Empirical vancomycin (VAN) treatment failure for methicillin-resistant (MRSA) bacteraemia, with significantly higher mortality, has been reported for MRSA strains with reduced VAN susceptibility..
View Article and Find Full Text PDFBackground: In recent years, the wearing of pierced earrings for personal adornment has increased among health care workers in Japan. However, the transmission dynamics between bacteria in pierced earring holes and fingers has not been clearly shown.
Methods: Earlobes and fingers of 200 nurses (128 nurses with pierced earlobes and 72 nurses with unpierced earlobes) working at a university hospital were sampled to determine whether cross-transmission of bacteria-colonized pierced earring holes and fingers in nurse is possible.
Objectives: Antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates obtained from 1996-2005 (n=200) and 2008-2016 (n=200) in Fukuoka, Japan, were examined.
Methods: MICs were determined by agar dilution. Sequence types (STs) were examined using N.
Article Synopsis
- * Using RNA extraction and quantitative PCR, we examined 13 specific genes to see how their expression changed with sitafloxacin treatment.
- * Results showed that a particular gene (lytA) had 3.9 times higher expression after just 10 minutes of exposure, indicating that sitafloxacin may rapidly induce these genes, contributing to its effectiveness against the bacteria.
Characterization of compensatory mutations associated with restoration of daptomycin-susceptibility in daptomycin non-susceptible methicillin-resistant Staphylococcus aureus and the role mprF mutations.
J Infect Chemother
January 2019
Article Synopsis
- The study aimed to understand how some clinically resistant MRSA strains become susceptible to Daptomycin (DAP) again by examining genomic and cell wall characteristics.
- Researchers found mutations in the mprF gene common in both non-susceptible and revertant strains, suggesting these mutations are not solely responsible for the reversion to DAP susceptibility.
- Differences in cell wall thickness were observed, with most strains showing increased thickness after DAP therapy and a subsequent reduction upon reverting to susceptibility, indicating that other factors also play a role in DAP resistance mechanisms.