Publications by authors named "Ajay Extross"
Adeno-associated virus (AAV) has emerged as a leading platform for gene therapy, enabling the delivery of therapeutic DNA to target cells. However, the potential of AAV to deliver protein payloads has been unexplored. In this study, we engineered a protein carrier AAV (pcAAV) to package and deliver proteins by inserting binding domains on the interior capsid surface.
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- AAV (Adeno-Associated Virus) is primarily used to deliver DNA, but researchers are exploring its potential to also deliver proteins through engineered capsids called Protein Carrier AAV (pcAAV).
- The study shows that inserting nanobodies into the AAV capsid allows for the packaging of various proteins, such as GFP and Cas9, and the efficiency of this process can be influenced by several design factors.
- The engineered proteins maintain their activity after being packaged and can effectively enter cells, suggesting that pcAAV could be a valuable new method for protein delivery in therapeutics and research.