Development of a novel tailor-made cocktail from recombinant crude enzymes for efficient saccharification of pretreated elephant grass.

A novel tailor-made enzyme cocktail comprising five recombinant crude enzymes was designed for hydrolysis of alkaline hydrogen peroxide pretreated elephant grass (AHPpEG). D-optimal design approach is employed to optimize the proportion of individual enzymes within the enzyme cocktail. The enzyme cocktail comprising chimera (endoglucanase and β-glucosidase), cellobiohydrolase, endo-1,4-β-xylanase, β-xylosidase and arabinofuranosidase having ratio (9:4:2:2:3) was developed.

A novel bifunctional type I α-l-arabinofuranosidase of family 43 glycoside hydrolase (BoGH43_35) from Bacteroides ovatus with endo-β-1,4-xylanase activity.

The gut bacterium Bacteroides ovatus harbors a diverse arsenal of glycoside hydrolases (GHs), which play pivotal roles in degrading dietary polysaccharides. In this study, we characterized a novel glycoside hydrolase from family 43 and subfamily 35 (BoGH43_35), cloned from B. ovatus.