Development of a real-time PCR and multiplex PCR assay for the detection and identification of mycotoxigenic fungi in stored maize grains.

This study aimed to identify important mycotoxigenic fungi and accurate detection of mycotoxin in stored maize grains using molecular methods. The current study also optimised the real-time PCR (RT-PCR) assay. The melting curve was established to identify isolated fungal species of (4), (3), (3), and (one).

An Eco-Friendly Approach Utilizing Green Synthesized Titanium Dioxide Nanoparticles for Leather Conservation against a Fungal Strain, AL1, Involved in the Biodeterioration of a Historical Manuscript.

The main hypothesis of the present research is investigating the efficacy of titanium oxide nanoparticles (TiO-NPs) to prevent the growth of fungal strains when applied on leather under an experimental study. Therefore, fifteen fungal strains were isolated from a deteriorated historical manuscript (papers and leathers) and identified by traditional methods and ITS sequence analysis, including (one isolate), (two strains), (four strains), (one strain), (one strain), (two strains), (two strains), and (two strains). The enzymes cellulase, amylase, pectinase, and gelatinase, which play a crucial role in biodegradation, were highly active in these fungal strains.

Comparison of Phytochemical Composition, Antibacterial, and Antifungal Activities of Extracts from Three Organs of from Saudi Arabia.

This in vitro study focused on the antimicrobial activity of methanolic and aqueous extracts of three organs (stems, roots, and leaves) of against nine bacterial species, two fungal, and one yeast strain. A comparative study of the yield, high-performance liquid chromatography (HPLC) composition, and polyphenol content of the different extracts was conducted. The obtained data showed that the yield of the methanolic extracts (between 13% and 33.

HPLC analysis, mycochemical contents and biological activities of two edible hypogeous ascomycetes: and .

Truffle, the hypogeous, ascomycetous macrofungus, has been recognized as a delicacy for centuries, and it is gaining elevated status in the culinary domain. The chemical composition and biological activities of aqueous extract and ground material of two desert Tunisian and were studied for the first time. Using three assays antioxidant activity of the aqueous extract and ground material of the two truffles were investigated.

Prevalence, antibiotic profile, virulence determinants, ESBLs, and non-β-lactam encoding genes of MDR spp. isolated from infected dogs.

This study investigated the prevalence, antibiogram, virulence, extended-spectrum β-lactamases (ESBLs), and non-β-lactam encoding genes of species isolated from infected dogs in Ismailia province, Egypt. The study was conducted on 70 fecal swabs collected from dogs with diarrhea for bacteriological identification of spp The positive isolates were evaluated for antibiotic susceptibility, molecular tests of virulence, ESBLs, and non-β-lactam encoding genes. Prevalence of spp.

Multilocus Sequence Typing and Antifungal Susceptibility of Isolates From Milk and Genital Tract of Dromedary Camel.

Multilocus sequence typing (MLST) was used to study the genetic diversity and population structure of 48 () isolates from the udder or genital tract of apparently healthy or diseased camels. This study aimed also to determine the frequency of isolates in the genital tract and udder of healthy or diseased female dromedary camels. A total of 240 mature dromedary camels (230 females and 10 males) were categorized based on the clinical examination of gentile tract and udder into five groups [fertile females ( = 70), infertile females ( = 115), healthy udder ( = 15), mastitis ( = 30), and fertile males ( = 10)].

Transcriptome Profiling Reveals Differential Gene Expression of Laccase Genes in KC462061 during Biodegradation of Crude Oil.

Fungal laccases have high catalytic efficiency and are utilized for the removal of crude oil because they oxidize various aliphatic and aromatic hydrocarbons and convert them into harmless compounds or less toxic compounds, thus accelerating the biodegradation potential of crude oil. Laccases are important gene families and the function of laccases genes varied widely based on transcription and function. Biodegradation of crude oil using KC462061 was studied in the current study beside the transcription level of eight laccase () genes have participated in biodegradation in the presence of aromatic compounds, and metal ions.