Large-Scale Chromatography for the Isolation of 7S Globulin Enriched Fraction from Pigeon Pea Seeds.

Pigeon pea () contains around 60% globulin proteins, of which 7S globulin is the most abundant fraction. In this work, a purification protocol for pigeon pea 7S globulin was developed using large-scale chromatography. The process was designed on an analytical scale through desalting of the crude protein extract followed by anion exchange chromatography and size exclusion chromatography.

Long-term storage of pink pepper essential oil microencapsulated by chickpea protein/pectin complexes: volatile release, antioxidant and antimicrobial activities.

Pink pepper essential oil was microencapsulated with chickpea protein (CP) and chickpea protein/pectin (CP-HMP) by spray drying. The reconstitution and storage properties of the powders were evaluated after drying. The impact of microencapsulation in the volatiles release, antioxidant and antimicrobial activity of oil was evaluated during 135 days of storage.

Modulation of volatile release and antimicrobial properties of pink pepper essential oil by microencapsulation in single- and double-layer structured matrices.

The bioactivity of essential oils applied in foods to act as natural preservatives can be reduced due to interactions with other components of the food matrix. Microencapsulation can help to increase the functionality of these compounds. In addition, the electrostatic interaction between proteins and polysaccharides can result in double-layered encapsulating structures, ensuring greater protection to essential oils than using only protein as surface active agent.